0 9 Inducible inducible JJ 10 16 nitric nitric JJ 17 22 oxide oxide NN 22 23 : : : 24 26 an an DT 27 41 autoregulatory autoregulatory JJ 42 50 feedback feedback NN 51 60 inhibitor inhibitor NN 61 63 of of IN 64 72 vascular vascular JJ 73 85 inflammation inflammation NN 85 86 . . . 88 97 Inducible inducible JJ 98 104 nitric nitric JJ 105 110 oxide oxide NN 111 112 ( ( ( 112 115 iNO ino NN 115 116 ) ) ) 117 119 is be VBZ 120 128 produced produce VBN 129 131 at at IN 132 137 sites site NNS 138 140 of of IN 141 149 vascular vascular JJ 150 162 inflammation inflammation NN 163 165 by by IN 166 174 resident resident JJ 175 178 and and CC 179 190 nonresident nonresident JJ 191 199 vascular vascular JJ 200 204 wall wall NN 205 210 cells cell NNS 210 211 , , , 212 215 but but CC 216 219 its its PRP$ 220 224 role role NN 225 227 in in IN 228 231 the the DT 232 244 inflammatory inflammatory JJ 245 252 process process NN 253 255 is be VBZ 256 259 not not RB 260 265 known know VBN 265 266 . . . 267 269 In in IN 270 274 this this DT 275 280 study study NN 280 281 , , , 282 284 we we PRP 285 289 show show VBP 290 294 that that IN 295 296 a a DT 297 302 novel novel JJ 303 311 function function NN 312 314 of of IN 315 318 iNO ino NN 319 321 is be VBZ 322 324 to to TO 325 334 terminate terminate VB 335 347 inflammatory inflammatory JJ 348 357 processes process NNS 357 358 . . . 359 361 We we PRP 362 366 find find VBP 367 371 that that IN 372 375 iNO ino NN 376 384 produced produce VBN 385 387 by by IN 388 394 murine murine JJ 395 410 macrophage-like macrophage-like JJ 411 416 cells cell NNS 416 417 , , , 418 426 RAW264.7 raw264.7 NN 426 427 , , , 428 431 can can MD 432 439 inhibit inhibit VB 440 456 cytokine-induced cytokine-induced JJ 457 468 endothelial endothelial JJ 469 473 cell cell NN 474 484 activation activation NN 485 487 in in IN 488 489 a a DT 490 499 separated separate VBN 500 503 and and CC 504 509 mixed mix VBN 510 530 endothelial-RAW264.7 endothelial-raw264.7 NN 531 540 coculture coculture NN 541 547 system system NN 547 548 . . . 549 553 Both both CC 554 557 iNO ino NN 558 568 production production NN 569 572 and and CC 573 584 endothelial endothelial JJ 585 591 VCAM-1 vcam-1 NN 592 602 expression expression NN 603 607 were be VBD 608 615 induced induce VBN 616 630 simultaneously simultaneously RB 631 635 with with IN 636 645 bacterial bacterial JJ 646 649 LPS lps NN 650 653 and and CC 654 669 murine-specific murine-specific JJ 670 679 IFN-gamma IFN-gamma NNP 679 680 . . . 681 691 Inhibition Inhibition NNP 692 694 of of IN 695 698 iNO ino NN 699 707 synthase synthase NN 708 709 ( ( ( 709 713 iNOS inos NN 713 714 ) ) ) 715 723 activity activity NN 724 728 with with IN 729 730 N N NNP 731 758 omega-monomethyl-L-arginine omega-monomethyl-l-arginine NN 759 761 in in IN 762 782 endothelial-RAW264.7 endothelial-raw264.7 NN 783 793 cocultures coculture NNS 793 794 , , , 795 805 stimulated stimulate VBN 806 810 with with IN 811 826 murine-specific murine-specific JJ 827 836 IFN-gamma IFN-gamma NNP 837 840 and and CC 841 844 LPS LPS NNP 844 845 , , , 846 855 decreased decrease VBD 856 859 iNO ino NN 860 870 production production NN 871 873 by by IN 874 876 86 86 CD 876 877 % % NN 877 878 , , , 879 888 augmented augment VBD 889 895 VCAM-1 vcam-1 NN 896 899 and and CC 900 904 iNOS inos NN 905 915 expression expression NN 916 918 in in IN 919 930 endothelial endothelial JJ 931 934 and and CC 935 943 RAW264.7 raw264.7 NN 944 949 cells cell NNS 949 950 , , , 951 963 respectively respectively RB 963 964 , , , 965 968 and and CC 969 978 increased increase VBD 979 987 monocyte monocyte NN 988 996 adhesion adhesion NN 997 999 to to TO 1000 1003 the the DT 1004 1015 endothelial endothelial JJ 1016 1020 cell cell NN 1021 1028 surface surface NN 1028 1029 . . . 1030 1039 Transient transient JJ 1040 1052 transfection transfection NN 1053 1060 studies study NNS 1061 1066 using use VBG 1067 1074 various various JJ 1075 1081 VCAM-1 vcam-1 NN 1082 1090 promoter promoter NN 1091 1101 constructs construct NNS 1102 1114 demonstrated demonstrate VBD 1115 1119 that that IN 1120 1130 inhibitory inhibitory JJ 1131 1138 effects effect NNS 1139 1141 of of IN 1142 1145 iNO ino NN 1146 1148 on on IN 1149 1155 VCAM-1 vcam-1 NN 1156 1160 gene gene NN 1161 1174 transcription transcription NN 1175 1179 were be VBD 1180 1188 mediated mediate VBN 1188 1189 , , , 1190 1192 in in IN 1193 1197 part part NN 1197 1198 , , , 1199 1201 by by IN 1202 1212 inhibitory inhibitory JJ 1213 1220 effects effect NNS 1221 1223 of of IN 1224 1227 iNO ino NN 1228 1230 on on IN 1231 1236 kappa kappa NN 1237 1238 B B NNP 1239 1249 cis-acting cis-acting JJ 1250 1258 elements element NNS 1258 1259 . . . 1260 1278 Immunofluorescence Immunofluorescence NNP 1279 1286 studies study NNS 1287 1292 using use VBG 1293 1295 an an DT 1296 1298 Ab Ab NNP 1299 1301 to to TO 1302 1305 the the DT 1306 1310 RelA RelA NNP 1311 1312 ( ( ( 1312 1315 p65 p65 NN 1315 1316 ) ) ) 1317 1324 subunit subunit NN 1325 1327 of of IN 1328 1335 nuclear nuclear JJ 1336 1348 factor-kappa factor-kappa NN 1349 1350 B B NNP 1351 1359 revealed reveal VBD 1360 1364 that that IN 1365 1368 iNO ino NN 1369 1378 inhibited inhibit VBD 1379 1382 the the DT 1383 1393 activation activation NN 1394 1396 of of IN 1397 1404 nuclear nuclear JJ 1405 1417 factor-kappa factor-kappa NN 1418 1420 B. B. NNP 1421 1426 These these DT 1427 1434 studies study NNS 1435 1443 indicate indicate VBP 1444 1448 that that IN 1449 1452 iNO ino NN 1453 1463 attenuates attenuate VBZ 1464 1468 iNOS inos NN 1469 1479 expression expression NN 1480 1482 in in IN 1483 1494 macrophages macrophage NNS 1495 1498 and and CC 1499 1507 inhibits inhibit VBZ 1508 1516 monocyte monocyte NN 1517 1525 adhesion adhesion NN 1526 1528 to to TO 1529 1540 endothelial endothelial JJ 1541 1546 cells cell NNS 1546 1547 , , , 1548 1551 and and CC 1552 1559 suggest suggest VBP 1560 1564 that that IN 1565 1577 endogenously endogenously RB 1578 1585 derived derive VBN 1586 1589 iNO ino NN 1590 1593 may may MD 1594 1596 be be VB 1597 1599 an an DT 1600 1609 important important JJ 1610 1624 autoregulatory autoregulatory JJ 1625 1634 inhibitor inhibitor NN 1635 1637 of of IN 1638 1646 vascular vascular JJ 1647 1659 inflammation inflammation NN 1659 1660 . . .