0 4 Role role NN 5 7 of of IN 8 11 the the DT 12 14 X2 x2 NN 15 18 box box NN 19 21 in in IN 22 31 activated activate VBN 32 45 transcription transcription NN 46 50 from from IN 51 54 the the DT 55 58 DRA DRA NNP 59 67 promoter promoter NN 68 70 in in IN 71 72 B B NNP 73 78 cells cell NNS 78 79 . . . 81 83 We we PRP 84 96 investigated investigate VBD 97 100 the the DT 101 109 function function NN 110 112 of of IN 113 116 the the DT 117 129 evolutionary evolutionary JJ 130 139 conserved conserve VBN 140 142 X2 x2 NN 143 146 box box NN 147 149 in in IN 150 153 the the DT 154 162 promoter promoter NN 163 165 of of IN 166 169 the the DT 170 177 HLA-DRA HLA-DRA NNP 178 182 gene gene NN 183 187 from from IN 188 191 the the DT 192 197 human human JJ 198 203 major major JJ 204 222 histocompatibility histocompatibility NN 223 230 complex complex NN 231 232 ( ( ( 232 235 MHC MHC NNP 235 236 ) ) ) 237 239 in in IN 240 247 resting rest VBG 248 251 and and CC 252 261 activated activate VBN 262 263 B B NNP 264 269 cells cell NNS 269 270 . . . 271 276 NF-X2 NF-X2 NNP 276 277 , , , 278 283 which which WDT 284 292 contains contain VBZ 293 300 members member NNS 301 303 of of IN 304 307 the the DT 308 321 AP-1/ATF/CREB AP-1/ATF/CREB NNP 322 330 families family NNS 331 333 of of IN 334 347 transcription transcription NN 348 355 factors factor NNS 355 356 , , , 357 366 interacts interact VBZ 367 371 with with IN 372 375 the the DT 376 378 X2 x2 NN 379 382 box box NN 383 384 ( ( ( 384 396 5’-TGCGTCA-3 5'-TGCGTCA-3 NNP 396 397 ’ ' '' 397 398 ) ) ) 399 403 from from IN 404 413 positions position NNS 414 417 -97 -97 CD 418 420 to to TO 421 424 -91 -91 CD 425 427 in in IN 428 431 the the DT 432 435 DRA DRA NNP 436 444 promoter promoter NN 444 445 . . . 446 448 In in IN 449 456 resting rest VBG 457 461 Raji Raji NNP 462 467 cells cell NNS 467 468 , , , 469 475 little little JJ 476 478 to to TO 479 481 no no DT 482 489 binding binding NN 490 492 to to TO 493 496 the the DT 497 499 X2 x2 NN 500 503 box box NN 504 507 was be VBD 508 516 observed observe VBN 516 517 . . . 518 520 In in IN 521 526 sharp sharp JJ 527 535 contrast contrast NN 535 536 , , , 537 539 in in IN 540 541 B B NNP 542 547 cells cell NNS 548 555 treated treat VBN 556 560 with with IN 561 564 the the DT 565 572 phorbol phorbol NN 573 578 ester ester NN 579 615 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate NNP 616 617 ( ( ( 617 620 TPA TPA NNP 620 621 ) ) ) 621 622 , , , 623 629 strong strong JJ 630 642 interactions interaction NNS 643 650 between between IN 651 654 the the DT 655 657 X2 x2 NN 658 661 box box NN 662 665 and and CC 666 671 NF-X2 nf-x2 NN 672 682 containing contain VBG 683 688 c-Fos c-fos NN 689 693 were be VBD 694 702 observed observe VBN 702 703 . . . 704 706 As as IN 707 717 determined determine VBN 718 720 by by IN 721 730 transient transient JJ 731 741 expression expression NN 742 745 and and CC 746 749 RNA RNA NNP 750 758 analyses analysis NNS 758 759 , , , 760 763 the the DT 764 774 activation activation NN 775 777 of of IN 778 785 protein protein NN 786 792 kinase kinase NNP 793 794 C C NNP 795 796 ( ( ( 796 799 PKC PKC NNP 799 800 ) ) ) 801 805 also also RB 806 815 increased increase VBD 816 821 rates rate NNS 822 824 of of IN 825 838 transcription transcription NN 839 843 from from IN 844 847 the the DT 848 857 wild-type wild-type JJ 858 861 DRA DRA NNP 862 870 promoter promoter NN 871 874 but but CC 875 878 not not RB 879 883 from from IN 884 885 a a DT 886 889 DRA DRA NNP 890 898 promoter promoter NN 899 906 bearing bear VBG 907 916 clustered cluster VBN 917 922 point point NN 923 932 mutations mutation NNS 933 935 in in IN 936 939 the the DT 940 942 X2 x2 NN 943 946 box box NN 946 947 . . . 948 953 Since since IN 954 957 the the DT 958 971 co-expression co-expression NN 972 976 with with IN 977 978 a a DT 979 987 dominant dominant JJ 988 996 negative negative JJ 997 1002 c-Fos c-fos NN 1003 1012 abolished abolish VBD 1013 1016 the the DT 1017 1031 responsiveness responsiveness NN 1032 1034 to to TO 1035 1038 TPA TPA NNP 1038 1039 , , , 1040 1042 we we PRP 1043 1051 conclude conclude VBP 1052 1056 that that IN 1057 1066 activated activate VBN 1067 1080 transcription transcription NN 1081 1083 of of IN 1084 1087 the the DT 1088 1091 DRA DRA NNP 1092 1096 gene gene NN 1097 1104 depends depend VBZ 1105 1107 on on IN 1108 1120 interactions interaction NNS 1121 1128 between between IN 1129 1132 the the DT 1133 1135 X2 x2 NN 1136 1139 box box NN 1140 1143 and and CC 1144 1149 NF-X2 nf-x2 NN 1149 1150 , , , 1151 1156 which which WDT 1157 1165 contains contain VBZ 1166 1171 c-Fos c-fos NN 1171 1172 . . .