0	8	Specific	specific	JJ
9	16	complex	complex	NN
17	26	formation	formation	NN
27	34	between	between	IN
35	38	the	the	DT
39	43	type	type	NN
44	46	II	ii	CD
47	51	bare	bare	JJ
52	62	lymphocyte	lymphocyte	NN
63	82	syndrome-associated	syndrome-associated	JJ
83	98	transactivators	transactivator	NNS
99	104	CIITA	CIITA	NNP
105	108	and	and	CC
109	113	RFX5	RFX5	NNP
113	114	.	.	.

116	119	Two	two	CD
120	122	of	of	IN
123	126	the	the	DT
127	132	genes	gene	NNS
133	142	defective	defective	JJ
143	145	in	in	IN
146	149	the	the	DT
150	154	five	five	CD
155	170	complementation	complementation	NN
171	177	groups	group	NNS
178	188	identified	identify	VBN
189	191	in	in	IN
192	195	the	the	DT
196	201	class	class	NN
202	213	II-negative	ii-negative	JJ
214	218	bare	bare	JJ
219	229	lymphocyte	lymphocyte	NN
230	238	syndrome	syndrome	NN
239	241	or	or	CC
242	255	corresponding	corresponding	JJ
256	266	laboratory	laboratory	NN
267	274	mutants	mutant	NNS
275	279	have	have	VBP
280	284	been	be	VBN
285	291	cloned	clone	VBN
291	292	.	.	.

293	296	One	one	CD
297	301	gene	gene	NN
302	309	encodes	encode	VBZ
310	311	a	a	DT
312	319	protein	protein	NN
319	320	,	,	,
321	325	RFX5	RFX5	NNP
325	326	,	,	,
327	331	that	that	WDT
332	334	is	be	VBZ
335	336	a	a	DT
337	343	member	member	NN
344	346	of	of	IN
347	350	the	the	DT
351	354	RFX	RFX	NNP
355	361	family	family	NN
362	364	of	of	IN
365	368	DNA	DNA	NNP
369	376	binding	binding	NN
377	385	proteins	protein	NNS
385	386	.	.	.

387	390	The	the	DT
391	396	other	other	JJ
396	397	,	,	,
398	403	CIITA	CIITA	NNP
403	404	,	,	,
405	412	encodes	encode	VBZ
413	414	a	a	DT
415	420	large	large	JJ
421	428	protein	protein	NN
429	433	with	with	IN
434	435	a	a	DT
436	443	defined	define	VBN
444	450	acidic	acidic	JJ
451	466	transcriptional	transcriptional	JJ
467	477	activation	activation	NN
478	484	domain	domain	NN
484	485	;	;	:
486	490	this	this	DT
491	498	protein	protein	NN
499	503	does	do	VBZ
504	507	not	not	RB
508	516	interact	interact	VB
517	521	with	with	IN
522	525	DNA	DNA	NNP
525	526	.	.	.

527	537	Expression	expression	NN
538	546	plasmids	plasmid	NNS
547	555	encoding	encode	VBG
556	563	regions	region	NNS
564	566	of	of	IN
567	571	RFX5	rfx5	NN
572	577	fused	fuse	VBN
578	580	to	to	TO
581	584	the	the	DT
585	589	GAL4	gal4	NN
590	593	DNA	DNA	NNP
594	601	binding	binding	NN
602	608	domain	domain	NN
609	618	activated	activate	VBD
619	632	transcription	transcription	NN
633	637	from	from	IN
638	639	a	a	DT
640	648	reporter	reporter	NN
649	658	construct	construct	NN
659	669	containing	contain	VBG
670	674	GAL4	gal4	NN
675	680	sites	site	NNS
681	683	in	in	IN
684	685	a	a	DT
686	700	cotransfection	cotransfection	NN
701	706	assay	assay	NN
707	709	in	in	IN
710	713	the	the	DT
714	718	Raji	Raji	NNP
719	724	human	human	JJ
725	726	B	B	NNP
727	731	cell	cell	NN
732	736	line	line	NN
736	737	.	.	.

738	745	However	however	RB
745	746	,	,	,
747	752	these	these	DT
753	761	plasmids	plasmid	NNS
762	770	produced	produce	VBD
771	786	transcriptional	transcriptional	JJ
787	795	activity	activity	NN
796	798	in	in	IN
799	803	HeLa	HeLa	NNP
804	809	cells	cell	NNS
810	814	only	only	RB
815	817	in	in	IN
818	829	conjunction	conjunction	NN
830	834	with	with	IN
835	845	interferon	interferon	NN
846	851	gamma	gamma	NN
852	863	stimulation	stimulation	NN
863	864	,	,	,
865	866	a	a	DT
867	876	condition	condition	NN
877	879	in	in	IN
880	885	which	which	WDT
886	896	expression	expression	NN
897	899	of	of	IN
900	904	both	both	CC
905	910	CIITA	CIITA	NNP
911	914	and	and	CC
915	920	class	class	NN
921	923	II	ii	CD
924	929	major	major	JJ
930	948	histocompatibility	histocompatibility	NN
949	956	complex	complex	NN
957	964	surface	surface	NN
965	973	proteins	protein	NNS
974	977	are	be	VBP
978	985	induced	induce	VBN
985	986	.	.	.

987	998	Furthermore	furthermore	RB
998	999	,	,	,
1000	1005	these	these	DT
1006	1014	plasmids	plasmid	NNS
1015	1019	were	be	VBD
1020	1023	not	not	RB
1024	1030	active	active	JJ
1031	1033	in	in	IN
1034	1041	RJ2.2.5	RJ2.2.5	NNP
1041	1042	,	,	,
1043	1045	an	an	DT
1046	1048	in	in	FW
1049	1054	vitro	vitro	FW
1055	1066	mutagenized	mutagenize	VBN
1067	1077	derivative	derivative	NN
1078	1080	of	of	IN
1081	1085	Raji	Raji	NNP
1086	1088	in	in	IN
1089	1094	which	which	WDT
1095	1099	both	both	DT
1100	1106	copies	copy	NNS
1107	1109	of	of	IN
1110	1115	CIITA	CIITA	NNP
1116	1119	are	be	VBP
1120	1129	defective	defective	JJ
1129	1130	.	.	.

1131	1146	Transcriptional	transcriptional	JJ
1147	1157	activation	activation	NN
1158	1160	by	by	IN
1161	1164	the	the	DT
1165	1169	RFX5	RFX5	NNP
1170	1176	fusion	fusion	NN
1177	1184	protein	protein	NN
1185	1190	could	could	MD
1191	1193	be	be	VB
1194	1202	restored	restore	VBN
1203	1205	in	in	IN
1206	1213	RJ2.2.5	rj2.2.5	NN
1214	1216	by	by	IN
1217	1231	cotransfection	cotransfection	NN
1232	1236	with	with	IN
1237	1238	a	a	DT
1239	1244	CIITA	CIITA	NNP
1245	1255	expression	expression	NN
1256	1263	plasmid	plasmid	NN
1263	1264	.	.	.

1265	1272	Finally	finally	RB
1272	1273	,	,	,
1274	1275	a	a	DT
1276	1282	direct	direct	JJ
1283	1294	interaction	interaction	NN
1295	1302	between	between	IN
1303	1307	RFX5	RFX5	NNP
1308	1311	and	and	CC
1312	1317	CIITA	CIITA	NNP
1318	1321	was	be	VBD
1322	1330	detected	detect	VBN
1331	1335	with	with	IN
1336	1339	the	the	DT
1340	1345	yeast	yeast	NN
1346	1356	two-hybrid	two-hybrid	JJ
1357	1360	and	and	CC
1361	1372	far-Western	far-western	JJ
1373	1377	blot	blot	NN
1378	1384	assays	assay	NNS
1384	1385	.	.	.

1386	1390	Thus	thus	RB
1390	1391	,	,	,
1392	1396	RFX5	rfx5	NN
1397	1400	can	can	MD
1401	1409	activate	activate	VB
1410	1423	transcription	transcription	NN
1424	1428	only	only	RB
1429	1431	in	in	IN
1432	1443	cooperation	cooperation	NN
1444	1448	with	with	IN
1449	1454	CIITA	CIITA	NNP
1454	1455	.	.	.

1456	1460	RFX5	RFX5	NNP
1461	1464	and	and	CC
1465	1470	CIITA	CIITA	NNP
1471	1480	associate	associate	VBP
1481	1483	to	to	TO
1484	1488	form	form	VB
1489	1490	a	a	DT
1491	1498	complex	complex	NN
1499	1506	capable	capable	JJ
1507	1509	of	of	IN
1510	1520	activating	activate	VBG
1521	1534	transcription	transcription	NN
1535	1539	from	from	IN
1540	1545	class	class	NN
1546	1548	II	ii	CD
1549	1554	major	major	JJ
1555	1573	histocompatibility	histocompatibility	NN
1574	1581	complex	complex	NN
1582	1591	promoters	promoter	NNS
1591	1592	.	.	.

1593	1595	In	in	IN
1596	1600	this	this	DT
1601	1608	complex	complex	NN
1608	1609	,	,	,
1610	1618	promoter	promoter	NN
1619	1630	specificity	specificity	NN
1631	1633	is	be	VBZ
1634	1644	determined	determine	VBN
1645	1647	by	by	IN
1648	1651	the	the	DT
1652	1655	DNA	dna	NN
1656	1663	binding	binding	NN
1664	1670	domain	domain	NN
1671	1673	of	of	IN
1674	1678	RFX5	RFX5	NNP
1679	1682	and	and	CC
1683	1686	the	the	DT
1687	1694	general	general	JJ
1695	1708	transcription	transcription	NN
1709	1718	apparatus	apparatus	NN
1719	1721	is	be	VBZ
1722	1731	recruited	recruit	VBN
1732	1734	by	by	IN
1735	1738	the	the	DT
1739	1745	acidic	acidic	JJ
1746	1756	activation	activation	NN
1757	1763	domain	domain	NN
1764	1766	of	of	IN
1767	1772	CIITA	CIITA	NNP
1772	1773	.	.	.