0 15 Transcriptional transcriptional JJ 16 25 induction induction NN 26 28 of of IN 29 42 collagenase-1 collagenase-1 NN 43 45 in in IN 46 60 differentiated differentiate VBN 61 74 monocyte-like monocyte-like JJ 75 76 ( ( ( 76 80 U937 u937 NN 80 81 ) ) ) 82 87 cells cell NNS 88 90 is be VBZ 91 100 regulated regulate VBN 101 103 by by IN 104 108 AP-1 ap-1 NN 109 112 and and CC 113 115 an an DT 116 124 upstream upstream JJ 125 135 C/EBP-beta C/EBP-beta NNP 136 140 site site NN 140 141 . . . 143 145 In in IN 146 150 this this DT 151 157 report report NN 157 158 , , , 159 161 we we PRP 162 173 demonstrate demonstrate VBP 174 178 that that IN 179 182 the the DT 183 187 AP-1 ap-1 NN 188 192 site site NN 193 196 and and CC 197 198 a a DT 199 205 distal distal JJ 206 214 promoter promoter NN 215 222 element element NN 223 231 regulate regulate VBP 232 247 transcriptional transcriptional JJ 248 257 induction induction NN 258 260 of of IN 261 274 collagenase-1 collagenase-1 NN 275 281 during during IN 282 291 monocytic monocytic JJ 292 307 differentiation differentiation NN 307 308 . . . 309 324 Chloramphenicol Chloramphenicol NNP 325 342 acetyltransferase acetyltransferase NN 343 353 expression expression NN 354 364 constructs construct NNS 365 375 containing contain VBG 376 383 regions region NNS 384 386 of of IN 387 390 the the DT 391 396 human human JJ 397 410 collagenase-1 collagenase-1 NN 411 419 promoter promoter NN 420 424 were be VBD 425 431 stably stably RB 432 434 or or CC 435 446 transiently transiently RB 447 458 transfected transfecte VBN 459 463 into into IN 464 468 U937 u937 NN 469 474 cells cell NNS 474 475 , , , 476 479 and and CC 480 488 reporter reporter NN 489 497 activity activity NN 498 501 was be VBD 502 510 assessed assess VBN 511 513 at at IN 514 521 various various JJ 522 527 times time NNS 528 533 after after IN 534 537 the the DT 538 543 onset onset NN 544 546 of of IN 547 554 phorbol phorbol NN 555 567 12-myristate 12-myristate NN 568 578 13-acetate 13-acetate NN 579 580 ( ( ( 580 593 PMA)-mediated pma)-mediated JJ 594 609 differentiation differentiation NN 609 610 . . . 611 616 Rapid rapid JJ 617 620 and and CC 621 627 strong strong JJ 628 637 induction induction NN 638 640 of of IN 641 649 promoter promoter NN 650 658 activity activity NN 659 662 was be VBD 663 667 lost lose VBN 668 670 in in IN 671 681 constructs construct NNS 682 686 with with IN 687 688 a a DT 689 695 mutant mutant JJ 696 700 AP-1 ap-1 NN 701 708 element element NN 708 709 ; ; : 710 717 however however RB 717 718 , , , 719 721 at at IN 722 727 16-96 16-96 CD 728 729 h h NN 730 738 post-PMA post-PMA NNP 738 739 , , , 740 743 the the DT 744 750 mutant mutant JJ 751 764 collagenase-1 collagenase-1 NN 765 773 promoter promoter NN 774 783 displayed display VBD 784 788 AP-1 ap-1 NN 789 800 independent independent JJ 801 813 PMA-mediated pma-mediated JJ 814 829 transactivation transactivation NN 829 830 . . . 831 834 The the DT 835 839 AP-1 ap-1 NN 840 846 mutant mutant JJ 847 857 constructs construct NNS 858 862 also also RB 863 869 showed show VBD 870 877 delayed delay VBN 878 893 transcriptional transcriptional JJ 894 904 activation activation NN 905 907 in in IN 908 919 PMA-treated pma-treated JJ 920 931 fibroblasts fibroblast NNS 931 932 . . . 933 940 Western western NN 941 944 and and CC 945 955 supershift supershift NN 956 964 analyses analysis NNS 965 974 indicated indicate VBD 975 979 that that IN 980 990 functional functional JJ 991 994 Jun Jun NNP 995 998 and and CC 999 1002 Fos Fos NNP 1003 1011 proteins protein NNS 1012 1016 were be VBD 1017 1024 present present JJ 1025 1027 in in IN 1028 1035 nuclear nuclear JJ 1036 1044 extracts extract NNS 1045 1047 of of IN 1048 1066 PMA-differentiated pma-differentiated JJ 1067 1071 U937 u937 NN 1072 1077 cells cell NNS 1077 1078 . . . 1079 1087 Promoter promoter NN 1088 1096 deletion deletion NN 1097 1107 constructs construct NNS 1108 1120 demonstrated demonstrate VBD 1121 1124 the the DT 1125 1134 potential potential JJ 1135 1139 role role NN 1140 1142 of of IN 1143 1149 distal distal JJ 1150 1158 promoter promoter NN 1159 1168 sequences sequence NNS 1169 1171 in in IN 1172 1182 regulating regulate VBG 1183 1196 collagenase-1 collagenase-1 NN 1197 1210 transcription transcription NN 1210 1211 . . . 1212 1214 In in IN 1215 1225 particular particular JJ 1225 1226 , , , 1227 1234 Western western JJ 1234 1235 , , , 1236 1246 supershift supershift NN 1246 1247 , , , 1248 1251 and and CC 1252 1260 promoter promoter NN 1261 1269 deletion deletion NN 1270 1278 analyses analysis NNS 1279 1288 suggested suggest VBD 1289 1290 a a DT 1291 1295 role role NN 1296 1299 for for IN 1300 1322 CCAAT/enhancer-binding ccaat/enhancer-binding JJ 1323 1335 protein-beta protein-beta NN 1336 1337 ( ( ( 1337 1347 C/EBP-beta C/EBP-beta NNP 1347 1348 ) ) ) 1349 1356 binding binding NN 1357 1361 site site NN 1362 1369 between between IN 1370 1375 -2010 -2010 CD 1376 1379 and and CC 1380 1385 -1954 -1954 CD 1386 1388 in in IN 1389 1399 regulating regulate VBG 1400 1413 transcription transcription NN 1414 1416 of of IN 1417 1430 collagenase-1 collagenase-1 NN 1431 1433 in in IN 1434 1443 monocytic monocytic JJ 1444 1449 cells cell NNS 1449 1450 . . . 1451 1454 Our our PRP$ 1455 1463 findings finding NNS 1464 1471 suggest suggest VBP 1472 1476 that that IN 1477 1485 distinct distinct JJ 1486 1496 regulatory regulatory JJ 1497 1505 elements element NNS 1505 1506 , , , 1507 1513 acting act VBG 1514 1522 somewhat somewhat RB 1523 1536 independently independently RB 1537 1539 of of IN 1540 1544 each each DT 1545 1550 other other JJ 1550 1551 , , , 1552 1559 control control JJ 1560 1570 expression expression NN 1571 1573 of of IN 1574 1587 collagenase-1 collagenase-1 NN 1587 1588 . . . 1589 1591 In in IN 1592 1600 addition addition NN 1600 1601 , , , 1602 1605 our our PRP$ 1606 1610 data datum NNS 1611 1619 suggests suggest VBZ 1620 1624 that that IN 1625 1628 the the DT 1629 1634 rapid rapid JJ 1635 1647 PMA-mediated pma-mediated JJ 1648 1657 induction induction NN 1658 1660 of of IN 1661 1674 collagenase-1 collagenase-1 NN 1675 1688 transcription transcription NN 1689 1691 is be VBZ 1692 1702 controlled control VBN 1703 1705 by by IN 1706 1707 a a DT 1708 1717 mechanism mechanism NN 1718 1726 distinct distinct JJ 1727 1731 from from IN 1732 1736 that that DT 1737 1747 regulating regulate VBG 1748 1751 the the DT 1752 1761 sustained sustained JJ 1762 1772 expression expression NN 1773 1775 of of IN 1776 1780 this this DT 1781 1791 proteinase proteinase NN 1792 1794 in in IN 1795 1804 activated activate VBN 1805 1816 macrophages macrophage NNS 1816 1817 . . .