0	11	Cooperation	Cooperation	NNP
12	19	between	between	IN
20	24	core	core	NN
25	32	binding	binding	NN
33	39	factor	factor	NN
40	43	and	and	CC
44	52	adjacent	adjacent	JJ
53	61	promoter	promoter	NN
62	70	elements	element	NNS
71	82	contributes	contribute	VBZ
83	85	to	to	TO
86	89	the	the	DT
90	105	tissue-specific	tissue-specific	JJ
106	116	expression	expression	NN
117	119	of	of	IN
120	133	interleukin-3	interleukin-3	NN
133	134	.	.	.

136	151	Tissue-specific	tissue-specific	JJ
152	162	expression	expression	NN
163	165	of	of	IN
166	179	interleukin-3	interleukin-3	NN
180	181	(	(	(
181	185	IL-3	il-3	NN
185	186	)	)	)
187	189	is	be	VBZ
190	198	mediated	mediate	VBN
199	202	via	via	IN
203	213	cis-acting	cis-acting	JJ
214	222	elements	element	NNS
223	230	located	located	JJ
231	237	within	within	IN
238	241	315	315	CD
242	246	base	base	NN
247	252	pairs	pair	NNS
253	255	of	of	IN
256	259	the	the	DT
260	273	transcription	transcription	NN
274	279	start	start	NN
279	280	.	.	.

281	285	This	this	DT
286	288	is	be	VBZ
289	297	achieved	achieve	VBN
298	300	in	in	IN
301	305	part	part	NN
306	313	through	through	IN
314	317	the	the	DT
318	326	positive	positive	JJ
327	337	activities	activity	NNS
338	340	of	of	IN
341	344	the	the	DT
345	349	AP-1	ap-1	NN
350	353	and	and	CC
354	359	Elf-1	elf-1	NN
360	365	sites	site	NNS
366	368	in	in	IN
369	372	the	the	DT
373	377	IL-3	il-3	NN
378	386	promoter	promoter	NN
386	387	.	.	.

388	391	The	the	DT
392	404	contribution	contribution	NN
405	407	to	to	TO
408	409	T	t	NN
410	423	cell-specific	cell-specific	JJ
424	434	expression	expression	NN
435	437	by	by	IN
438	443	other	other	JJ
444	452	promoter	promoter	NN
453	458	sites	site	NNS
459	462	was	be	VBD
463	471	assessed	assess	VBN
472	474	in	in	IN
475	476	a	a	DT
477	486	transient	transient	JJ
487	497	expression	expression	NN
498	503	assay	assay	NN
504	508	with	with	IN
509	513	IL-3	il-3	NN
514	522	promoter	promoter	NN
523	533	constructs	construct	NNS
534	540	linked	link	VBN
541	543	to	to	TO
544	545	a	a	DT
546	556	luciferase	luciferase	NN
557	561	gene	gene	NN
561	562	,	,	,
563	571	focusing	focus	VBG
572	581	initially	initially	RB
582	584	on	on	IN
585	588	the	the	DT
589	593	core	core	NN
594	601	binding	binding	NN
602	608	factor	factor	NN
609	610	(	(	(
610	613	CBF	CBF	NNP
613	614	)	)	)
615	619	site	site	NN
619	620	,	,	,
621	626	which	which	WDT
627	629	is	be	VBZ
630	641	footprinted	footprinte	VBN
642	644	in	in	FW
645	649	vivo	vivo	FW
650	654	upon	upon	IN
655	656	T	t	NN
657	661	cell	cell	NN
662	672	activation	activation	NN
672	673	.	.	.

674	682	Activity	activity	NN
683	685	of	of	IN
686	689	the	the	DT
690	693	CBF	CBF	NNP
694	698	site	site	NN
699	701	is	be	VBZ
702	707	shown	show	VBN
708	710	to	to	TO
711	713	be	be	VB
714	724	critically	critically	RB
725	734	dependent	dependent	JJ
735	737	on	on	IN
738	741	the	the	DT
742	750	adjacent	adjacent	JJ
751	760	activator	activator	NN
761	765	site	site	NN
766	771	Act-1	act-1	NN
771	772	.	.	.

773	781	Together	together	RB
782	785	the	the	DT
786	791	Act-1	act-1	NN
792	795	and	and	CC
796	799	CBF	cbf	NN
800	805	sites	site	NNS
806	810	form	form	VBP
811	812	a	a	DT
813	823	functional	functional	JJ
824	828	unit	unit	NN
829	830	(	(	(
830	832	AC	AC	NNP
833	837	unit	unit	NN
837	838	)	)	)
839	843	with	with	IN
844	848	dual	dual	JJ
849	857	activity	activity	NN
857	858	.	.	.

859	862	The	the	DT
863	865	AC	AC	NNP
866	870	unit	unit	NN
871	873	is	be	VBZ
874	886	demonstrated	demonstrate	VBN
887	889	to	to	TO
890	897	enhance	enhance	VB
898	903	basal	basal	JJ
904	912	activity	activity	NN
913	915	of	of	IN
916	925	promoters	promoter	NNS
926	930	both	both	CC
931	933	in	in	IN
934	945	fibroblasts	fibroblast	NNS
946	949	and	and	CC
950	951	T	t	NN
952	957	cells	cell	NNS
957	958	.	.	.

959	963	This	this	DT
964	972	activity	activity	NN
973	975	is	be	VBZ
976	983	further	further	RBR
984	993	inducible	inducible	JJ
994	996	in	in	IN
997	1006	activated	activate	VBN
1007	1008	T	t	NN
1009	1014	cells	cell	NNS
1014	1015	,	,	,
1016	1019	but	but	CC
1020	1023	not	not	RB
1024	1026	in	in	IN
1027	1038	fibroblasts	fibroblast	NNS
1038	1039	.	.	.

1040	1042	In	in	IN
1043	1051	addition	addition	NN
1052	1054	to	to	TO
1055	1058	the	the	DT
1059	1066	already	already	RB
1067	1077	identified	identify	VBN
1078	1081	NIP	NIP	NNP
1082	1091	repressor	repressor	NN
1092	1096	site	site	NN
1096	1097	,	,	,
1098	1106	evidence	evidence	NN
1107	1109	is	be	VBZ
1110	1119	presented	present	VBN
1120	1123	for	for	IN
1124	1125	a	a	DT
1126	1132	second	second	JJ
1133	1142	repressor	repressor	NN
1143	1149	region	region	NN
1150	1154	that	that	WDT
1155	1164	restricts	restrict	VBZ
1165	1173	promoter	promoter	NN
1174	1182	activity	activity	NN
1183	1185	in	in	IN
1186	1197	fibroblasts	fibroblast	NNS
1197	1198	.	.	.

1199	1206	Finally	finally	RB
1206	1207	,	,	,
1208	1209	a	a	DT
1210	1215	novel	novel	JJ
1216	1224	positive	positive	JJ
1225	1235	regulatory	regulatory	JJ
1236	1243	element	element	NN
1244	1247	has	have	VBZ
1248	1252	been	be	VBN
1253	1259	mapped	map	VBN
1260	1262	in	in	IN
1263	1266	the	the	DT
1267	1271	IL-3	il-3	NN
1272	1280	promoter	promoter	NN
1281	1288	between	between	IN
1289	1299	nucleotide	nucleotide	NN
1300	1304	-180	-180	CD
1305	1308	and	and	CC
1309	1313	-210	-210	CD
1314	1318	that	that	WDT
1319	1324	leads	lead	VBZ
1325	1327	to	to	TO
1328	1337	increased	increase	VBN
1338	1348	expression	expression	NN
1349	1351	in	in	IN
1352	1353	T	t	NN
1354	1359	cells	cell	NNS
1359	1360	.	.	.

1361	1369	Together	together	RB
1370	1375	these	these	DT
1376	1383	results	result	NNS
1384	1395	demonstrate	demonstrate	VBP
1396	1400	that	that	IN
1401	1402	T	t	NN
1403	1407	cell	cell	NN
1408	1418	expression	expression	NN
1419	1421	of	of	IN
1422	1426	IL-3	IL-3	NNP
1427	1429	is	be	VBZ
1430	1433	not	not	RB
1434	1443	specified	specify	VBN
1444	1446	by	by	IN
1447	1450	the	the	DT
1451	1459	activity	activity	NN
1460	1462	of	of	IN
1463	1464	a	a	DT
1465	1471	single	single	JJ
1472	1487	tissue-specific	tissue-specific	JJ
1488	1495	element	element	NN
1495	1496	,	,	,
1497	1500	but	but	CC
1501	1508	instead	instead	RB
1509	1517	involves	involve	VBZ
1518	1526	multiple	multiple	JJ
1527	1538	interacting	interact	VBG
1539	1547	elements	element	NNS
1548	1552	that	that	WDT
1553	1560	provide	provide	VBP
1561	1565	both	both	CC
1566	1574	specific	specific	JJ
1575	1583	positive	positive	JJ
1584	1594	regulation	regulation	NN
1595	1597	in	in	IN
1598	1599	T	t	NN
1600	1605	cells	cell	NNS
1606	1609	and	and	CC
1610	1618	specific	specific	JJ
1619	1627	negative	negative	JJ
1628	1638	regulation	regulation	NN
1639	1641	in	in	IN
1642	1653	fibroblasts	fibroblast	NNS
1653	1654	.	.	.