0 10 Expression expression NN 11 13 of of IN 14 19 v-src v-src NN 20 22 in in IN 23 24 T t NN 25 30 cells cell NNS 31 41 correlates correlate VBZ 42 46 with with IN 47 54 nuclear nuclear JJ 55 65 expression expression NN 66 68 of of IN 69 77 NF-kappa NF-kappa NNP 78 80 B. B. NNP 82 90 NF-kappa NF-kappa NNP 91 92 B B NNP 93 95 is be VBZ 96 97 a a DT 98 105 rapidly rapidly RB 106 115 inducible inducible JJ 116 131 transcriptional transcriptional JJ 132 141 activator activator NN 142 146 that that WDT 147 155 responds respond VBZ 156 158 to to TO 159 160 a a DT 161 168 variety variety NN 169 171 of of IN 172 179 signals signal NNS 180 183 and and CC 184 194 influences influence VBZ 195 198 the the DT 199 209 expression expression NN 210 212 of of IN 213 217 many many JJ 218 223 genes gene NNS 224 232 involved involve VBN 233 235 in in IN 236 239 the the DT 240 246 immune immune JJ 247 255 response response NN 255 256 . . . 257 264 Protein protein NN 265 273 tyrosine tyrosine NN 274 281 kinases kinase NNS 282 290 transmit transmit VBP 291 298 signals signal NNS 299 303 from from IN 304 312 cytokine cytokine NN 313 316 and and CC 317 323 immune immune JJ 324 333 receptors receptor NNS 333 334 . . . 335 339 Very very RB 340 346 little little JJ 347 358 information information NN 359 365 exists exist VBZ 366 373 linking link VBG 374 379 these these DT 380 383 two two CD 384 393 important important JJ 394 401 classes class NNS 402 404 of of IN 405 414 signaling signal VBG 415 424 molecules molecule NNS 424 425 . . . 426 428 We we PRP 429 432 now now RB 433 444 demonstrate demonstrate VBP 445 449 that that IN 450 455 v-src v-src NN 456 466 expression expression NN 467 477 correlates correlate VBZ 478 482 with with IN 483 490 nuclear nuclear JJ 491 501 expression expression NN 502 504 of of IN 505 506 a a DT 507 512 kappa kappa NN 513 514 B B NNP 515 522 binding binding NN 523 530 complex complex NN 531 538 similar similar JJ 539 541 to to TO 542 546 that that DT 547 554 induced induce VBN 555 557 by by IN 558 565 phorbol phorbol NN 566 571 ester ester NN 572 575 and and CC 576 585 ionomycin ionomycin NN 585 586 , , , 587 589 as as IN 590 598 detected detect VBN 599 601 by by IN 602 617 electrophoretic electrophoretic JJ 618 626 mobility mobility NN 627 632 shift shift NN 633 638 assay assay NN 639 644 using use VBG 645 646 a a DT 647 654 variety variety NN 655 657 of of IN 658 663 kappa kappa NN 664 665 B B NNP 666 671 sites site NNS 671 672 . . . 673 677 This this DT 678 685 complex complex NN 686 689 was be VBD 690 697 blocked block VBN 698 700 by by IN 701 704 the the DT 705 713 tyrosine tyrosine NN 714 720 kinase kinase NNP 721 730 inhibitor inhibitor NN 730 731 , , , 732 742 herbimycin herbimycin NN 743 744 A a NN 744 745 . . . 746 749 The the DT 750 763 v-src-induced v-src-induced JJ 764 771 complex complex NN 772 781 comprised comprise VBD 782 785 the the DT 786 789 p50 p50 NN 790 793 and and CC 794 797 p65 p65 NN 798 808 components component NNS 809 811 of of IN 812 820 NF-kappa NF-kappa NNP 821 822 B B NNP 822 823 , , , 824 826 as as IN 827 837 determined determine VBN 838 840 by by IN 841 851 supershift supershift NN 852 855 and and CC 856 866 immunoblot immunoblot NN 867 875 analysis analysis NN 875 876 . . . 877 879 As as IN 880 881 a a DT 882 892 functional functional JJ 893 902 correlate correlate NN 903 905 of of IN 906 910 this this DT 911 918 finding finding NN 918 919 , , , 920 929 transient transient JJ 930 945 co-transfection co-transfection NN 946 948 of of IN 949 954 HIV-1 HIV-1 NNP 955 958 LTR LTR NNP 959 967 reporter reporter NN 968 978 constructs construct NNS 979 981 in in IN 982 983 a a DT 984 993 different different JJ 994 995 T t NN 996 1000 cell cell NN 1001 1005 line line NN 1006 1018 demonstrated demonstrate VBD 1019 1023 that that IN 1024 1029 v-src v-src NN 1030 1039 activated activate VBD 1040 1044 this this DT 1045 1053 promoter promoter NN 1054 1056 in in IN 1057 1058 a a DT 1059 1064 kappa kappa NN 1065 1076 B-dependent b-dependent JJ 1077 1083 manner manner NN 1083 1084 . . . 1085 1087 We we PRP 1088 1093 found find VBD 1094 1098 that that IN 1099 1114 transactivation transactivation NN 1115 1117 of of IN 1118 1121 the the DT 1122 1127 HIV-1 HIV-1 NNP 1128 1131 LTR LTR NNP 1132 1134 by by IN 1135 1140 v-src v-src NN 1141 1144 was be VBD 1145 1149 more more RBR 1150 1159 sensitive sensitive JJ 1160 1162 to to TO 1163 1172 mutations mutation NNS 1173 1175 of of IN 1176 1179 the the DT 1180 1188 proximal proximal JJ 1188 1189 , , , 1190 1196 rather rather RB 1197 1201 than than IN 1202 1205 the the DT 1206 1212 distal distal JJ 1212 1213 , , , 1214 1219 kappa kappa NN 1220 1221 B B NNP 1222 1229 element element NN 1229 1230 . . . 1231 1234 The the DT 1235 1247 implications implication NNS 1248 1251 for for IN 1252 1253 T t NN 1254 1258 cell cell NN 1259 1267 receptor receptor NN 1268 1277 signaling signaling NN 1278 1281 and and CC 1282 1287 HIV-1 HIV-1 NNP 1288 1292 gene gene NN 1293 1303 expression expression NN 1304 1307 are be VBP 1308 1318 considered consider VBN 1318 1319 . . .