0	9	Long-term	long-term	JJ
10	18	inositol	inositol	NN
19	28	phosphate	phosphate	NN
29	36	release	release	NN
36	37	,	,	,
38	41	but	but	CC
42	45	not	not	RB
46	54	tyrosine	tyrosine	NN
55	61	kinase	kinase	NNP
62	70	activity	activity	NN
70	71	,	,	,
72	82	correlates	correlate	VBZ
83	87	with	with	IN
88	92	IL-2	il-2	NN
93	102	secretion	secretion	NN
103	106	and	and	CC
107	112	NF-AT	nf-at	NN
113	122	induction	induction	NN
123	125	in	in	IN
126	144	anti-CD3-activated	anti-cd3-activated	JJ
145	155	peripheral	peripheral	JJ
156	161	human	human	JJ
162	163	T	t	NN
164	175	lymphocytes	lymphocyte	NNS
175	176	.	.	.

178	181	The	the	DT
182	189	cascade	cascade	NN
190	192	of	of	IN
193	199	events	event	NNS
200	206	within	within	IN
207	210	the	the	DT
211	216	first	first	JJ
217	220	few	few	JJ
221	228	minutes	minute	NNS
229	231	of	of	IN
232	233	T	t	NN
234	238	cell	cell	NN
239	250	stimulation	stimulation	NN
251	254	has	have	VBZ
255	259	been	be	VBN
260	264	well	well	RB
265	278	characterized	characterize	VBN
278	279	.	.	.

280	288	Although	although	IN
289	293	many	many	JJ
294	300	second	second	JJ
301	311	messengers	messenger	NNS
312	316	have	have	VBP
317	321	been	be	VBN
322	327	shown	show	VBN
328	330	to	to	TO
331	333	be	be	VB
334	343	necessary	necessary	JJ
344	347	and	and	CC
348	358	sufficient	sufficient	JJ
359	362	for	for	IN
363	364	T	t	NN
365	369	cell	cell	NN
370	380	activation	activation	NN
381	383	in	in	IN
384	385	a	a	DT
386	392	number	number	NN
393	395	of	of	IN
396	401	model	model	NN
402	409	systems	system	NNS
409	410	,	,	,
411	414	the	the	DT
415	428	rate-limiting	rate-limiting	JJ
429	433	step	step	NN
434	436	in	in	IN
437	447	peripheral	peripheral	JJ
448	449	T	t	NN
450	455	cells	cell	NNS
456	459	has	have	VBZ
460	463	not	not	RB
464	468	been	be	VBN
469	481	demonstrated	demonstrate	VBN
481	482	.	.	.

483	485	To	to	TO
486	491	model	model	VB
492	501	effective	effective	JJ
502	508	versus	versus	IN
509	520	ineffective	ineffective	JJ
521	533	CD3-mediated	cd3-mediated	JJ
534	545	stimulation	stimulation	NN
546	548	in	in	IN
549	559	peripheral	peripheral	JJ
560	561	T	t	NN
562	567	cells	cell	NNS
567	568	,	,	,
569	571	we	we	PRP
572	576	used	use	VBD
577	580	two	two	CD
581	589	anti-CD3	anti-cd3	JJ
590	594	mAbs	mab	NNS
595	599	that	that	WDT
600	606	differ	differ	VBP
607	609	in	in	IN
610	615	their	their	PRP$
616	623	ability	ability	NN
624	626	to	to	TO
627	636	stimulate	stimulate	VB
637	645	purified	purify	VBN
646	647	T	t	NN
648	653	cells	cell	NNS
653	654	:	:	:
655	659	OKT3	OKT3	NNP
659	660	,	,	,
661	666	which	which	WDT
667	673	causes	cause	VBZ
674	679	early	early	JJ
680	686	second	second	JJ
687	696	messenger	messenger	NN
697	707	generation	generation	NN
708	711	but	but	CC
712	714	is	be	VBZ
715	721	unable	unable	JJ
722	724	to	to	TO
725	733	activate	activate	VB
734	735	T	t	NN
736	741	cells	cell	NNS
742	749	without	without	IN
750	751	a	a	DT
752	758	second	second	JJ
759	765	signal	signal	NN
765	766	,	,	,
767	770	and	and	CC
771	775	64.1	64.1	CD
775	776	,	,	,
777	782	which	which	WDT
783	793	stimulates	stimulate	VBZ
794	795	T	t	NN
796	800	cell	cell	NN
801	814	proliferation	proliferation	NN
815	817	on	on	IN
818	821	its	its	PRP$
822	825	own	own	JJ
825	826	.	.	.

827	829	We	we	PRP
830	835	found	find	VBD
836	840	that	that	IN
841	849	tyrosine	tyrosine	NN
850	856	kinase	kinase	NNP
857	865	activity	activity	NN
866	869	was	be	VBD
870	877	similar	similar	JJ
878	881	for	for	IN
882	886	both	both	DT
887	891	mAbs	mab	NNS
892	896	over	over	IN
897	898	a	a	DT
899	905	period	period	NN
906	908	of	of	IN
909	914	hours	hour	NNS
914	915	.	.	.

916	923	However	however	RB
923	924	,	,	,
925	928	the	the	DT
929	937	inositol	inositol	NN
938	947	phosphate	phosphate	NN
948	956	response	response	NN
957	960	was	be	VBD
961	969	stronger	strong	JJR
970	973	for	for	IN
974	978	64.1	64.1	CD
979	983	than	than	IN
984	987	for	for	IN
988	992	OKT3	OKT3	NNP
992	993	.	.	.

994	996	To	to	TO
997	1000	tie	tie	VB
1001	1006	these	these	DT
1007	1013	events	event	NNS
1014	1016	to	to	TO
1017	1021	gene	gene	NN
1022	1032	activation	activation	NN
1032	1033	,	,	,
1034	1036	we	we	PRP
1037	1045	measured	measure	VBD
1046	1054	NF-kappa	NF-kappa	NNP
1055	1056	B	B	NNP
1057	1060	and	and	CC
1061	1066	NF-AT	NF-AT	NNP
1067	1075	activity	activity	NN
1076	1078	in	in	IN
1079	1082	the	the	DT
1083	1090	nucleus	nucleus	NN
1091	1096	after	after	IN
1097	1105	anti-CD3	anti-cd3	JJ
1106	1117	stimulation	stimulation	NN
1117	1118	.	.	.

1119	1123	Both	both	DT
1124	1131	stimuli	stimulus	NNS
1132	1139	induced	induce	VBD
1140	1143	the	the	DT
1144	1154	appearance	appearance	NN
1155	1157	of	of	IN
1158	1161	the	the	DT
1162	1170	NF-kappa	NF-kappa	NNP
1171	1172	B	B	NNP
1173	1183	components	component	NNS
1184	1185	(	(	(
1185	1190	c-Rel	c-rel	NN
1190	1191	,	,	,
1192	1195	p65	p65	NN
1196	1197	(	(	(
1197	1201	RelA	RelA	NNP
1201	1202	)	)	)
1202	1203	,	,	,
1204	1207	and	and	CC
1208	1211	p50	p50	NN
1212	1213	(	(	(
1213	1221	NF-kappa	NF-kappa	NNP
1222	1224	B1	B1	NNP
1224	1225	)	)	)
1225	1226	)	)	)
1227	1230	and	and	CC
1231	1239	NF-kappa	NF-kappa	NNP
1240	1241	B	B	NNP
1242	1245	DNA	DNA	NNP
1246	1253	binding	binding	NN
1254	1262	activity	activity	NN
1263	1265	in	in	IN
1266	1269	the	the	DT
1270	1277	nucleus	nucleus	NN
1277	1278	.	.	.

1279	1286	However	however	RB
1286	1287	,	,	,
1288	1292	only	only	RB
1293	1297	64.1	64.1	CD
1298	1305	induced	induce	VBD
1306	1311	NF-AT	nf-at	NN
1312	1314	in	in	IN
1315	1318	the	the	DT
1319	1326	nucleus	nucleus	NN
1326	1327	,	,	,
1328	1339	correlating	correlate	VBG
1340	1344	with	with	IN
1345	1348	its	its	PRP$
1349	1356	ability	ability	NN
1357	1359	to	to	TO
1360	1368	activate	activate	VB
1369	1370	T	t	NN
1371	1376	cells	cell	NNS
1376	1377	.	.	.

1378	1382	Thus	thus	RB
1382	1383	,	,	,
1384	1389	NF-AT	NF-AT	NNP
1390	1399	induction	induction	NN
1400	1403	and	and	CC
1404	1408	IL-2	il-2	NN
1409	1418	secretion	secretion	NN
1419	1423	were	be	VBD
1424	1434	correlated	correlate	VBN
1435	1439	with	with	IN
1440	1443	the	the	DT
1444	1450	levels	level	NNS
1451	1453	of	of	IN
1454	1462	inositol	inositol	NN
1463	1472	phosphate	phosphate	NN
1473	1480	release	release	NN
1481	1484	but	but	CC
1485	1488	not	not	RB
1489	1493	with	with	IN
1494	1499	gross	gross	JJ
1500	1506	levels	level	NNS
1507	1509	of	of	IN
1510	1518	tyrosine	tyrosine	NN
1519	1525	kinase	kinase	NNP
1526	1534	activity	activity	NN
1535	1542	induced	induce	VBN
1543	1547	late	late	RB
1548	1557	following	follow	VBG
1558	1561	the	the	DT
1562	1570	response	response	NN
1570	1571	.	.	.

1572	1574	On	on	IN
1575	1578	the	the	DT
1579	1584	other	other	JJ
1585	1589	hand	hand	NN
1589	1590	,	,	,
1591	1599	NF-kappa	NF-kappa	NNP
1600	1601	B	B	NNP
1602	1611	induction	induction	NN
1612	1615	and	and	CC
1616	1620	IL-2	il-2	NN
1621	1629	receptor	receptor	NN
1630	1640	expression	expression	NN
1641	1649	occurred	occur	VBD
1650	1654	even	even	RB
1655	1659	with	with	IN
1660	1663	the	the	DT
1664	1671	smaller	small	JJR
1672	1678	second	second	JJ
1679	1688	messenger	messenger	NN
1689	1697	response	response	NN
1698	1707	generated	generate	VBN
1708	1710	by	by	IN
1711	1715	OKT3	OKT3	NNP
1715	1716	.	.	.