0 10 Regulation regulation NN 11 13 of of IN 14 32 cell-type-specific cell-type-specific JJ 33 46 interleukin-2 interleukin-2 NN 47 55 receptor receptor NN 56 67 alpha-chain alpha-chain NN 68 72 gene gene NN 73 83 expression expression NN 83 84 : : : 85 94 potential potential JJ 95 99 role role NN 100 102 of of IN 103 111 physical physical JJ 112 124 interactions interaction NNS 125 132 between between IN 133 138 Elf-1 Elf-1 NNP 138 139 , , , 140 148 HMG-I(Y) hmg-i(y) NN 148 149 , , , 150 153 and and CC 154 162 NF-kappa NF-kappa NNP 163 164 B B NNP 165 171 family family NN 172 180 proteins protein NNS 180 181 . . . 183 186 The the DT 187 198 interleukin interleukin NN 199 200 2 2 CD 201 209 receptor receptor NN 210 221 alpha-chain alpha-chain NN 222 223 ( ( ( 223 228 IL-2R IL-2R NNP 229 234 alpha alpha NN 234 235 ) ) ) 236 240 gene gene NN 241 243 is be VBZ 244 251 rapidly rapidly RB 252 255 and and CC 256 264 potently potently RB 265 272 induced induce VBN 273 275 in in IN 276 277 T t NN 278 283 cells cell NNS 284 286 in in IN 287 295 response response NN 296 298 to to TO 299 308 mitogenic mitogenic JJ 309 316 stimuli stimulus NNS 316 317 . . . 318 328 Previously previously RB 328 329 , , , 330 332 an an DT 333 342 inducible inducible JJ 343 351 enhancer enhancer NN 352 359 between between IN 360 371 nucleotides nucleotide NNS 372 376 -299 -299 CD 377 380 and and CC 381 385 -228 -228 CD 386 390 that that WDT 391 399 contains contain VBZ 400 408 NF-kappa NF-kappa NNP 409 410 B B NNP 411 414 and and CC 415 419 CArG CArG NNP 420 426 motifs motif NNS 427 430 was be VBD 431 441 identified identify VBN 441 442 . . . 443 445 We we PRP 446 449 now now RB 450 456 report report VBP 457 460 the the DT 461 477 characterization characterization NN 478 480 of of IN 481 482 a a DT 483 489 second second JJ 490 499 essential essential JJ 500 508 positive positive JJ 509 519 regulatory regulatory JJ 520 527 element element NN 528 535 located located JJ 536 543 between between IN 544 555 nucleotides nucleotide NNS 556 560 -137 -137 CD 561 564 and and CC 565 568 -64 -64 CD 569 573 that that WDT 574 579 binds bind VBZ 580 585 Elf-1 elf-1 NN 586 589 and and CC 590 598 HMG-I(Y) HMG-I(Y) NNP 598 599 . . . 600 604 This this DT 605 612 element element NN 613 616 had have VBD 617 624 maximal maximal JJ 625 633 activity activity NN 634 636 in in IN 637 645 lymphoid lymphoid JJ 646 651 cells cell NNS 651 652 , , , 653 664 paralleling parallel VBG 665 668 the the DT 669 673 cell cell NN 674 678 type type NN 679 690 specificity specificity NN 691 693 of of IN 694 699 Elf-1 elf-1 NN 700 710 expression expression NN 710 711 . . . 712 725 Transcription transcription NN 726 730 from from IN 731 734 the the DT 735 740 IL-2R IL-2R NNP 741 746 alpha alpha NN 747 755 promoter promoter NN 756 759 was be VBD 760 769 inhibited inhibit VBN 770 774 when when WRB 775 781 either either CC 782 785 the the DT 786 791 Elf-1 elf-1 NN 792 794 or or CC 795 798 the the DT 799 807 HMG-I(Y) hmg-i(y) NN 808 815 binding binding NN 816 820 site site NN 821 824 was be VBD 825 832 mutated mutate VBN 832 833 . . . 834 846 Coexpression coexpression NN 847 849 of of IN 850 854 both both DT 855 863 proteins protein NNS 864 873 activated activate VBD 874 887 transcription transcription NN 888 890 of of IN 891 894 the the DT 895 899 -137 -137 CD 900 902 to to TO 903 906 -64 -64 CD 907 914 element element NN 915 917 in in IN 918 923 COS-7 cos-7 NN 924 929 cells cell NNS 929 930 . . . 931 936 Elf-1 Elf-1 NNP 937 947 physically physically RB 948 958 associated associate VBN 959 963 with with IN 964 969 HMG-I HMG-I NNP 970 973 and and CC 974 978 with with IN 979 987 NF-kappa NF-kappa NNP 988 989 B B NNP 990 993 p50 p50 NN 994 997 and and CC 998 1003 c-Rel c-rel NN 1004 1006 in in FW 1007 1012 vitro vitro FW 1012 1013 , , , 1014 1024 suggesting suggest VBG 1025 1029 that that IN 1030 1045 protein-protein protein-protein JJ 1046 1058 interactions interaction NNS 1059 1064 might might MD 1065 1077 functionally functionally RB 1078 1088 coordinate coordinate VB 1089 1092 the the DT 1093 1100 actions action NNS 1101 1103 of of IN 1104 1107 the the DT 1108 1116 upstream upstream JJ 1117 1120 and and CC 1121 1131 downstream downstream JJ 1132 1140 positive positive JJ 1141 1151 regulatory regulatory JJ 1152 1160 elements element NNS 1160 1161 . . . 1162 1166 This this DT 1167 1169 is be VBZ 1170 1173 the the DT 1174 1179 first first JJ 1180 1186 report report NN 1187 1189 of of IN 1190 1191 a a DT 1192 1200 physical physical JJ 1201 1212 interaction interaction NN 1213 1220 between between IN 1221 1223 an an DT 1224 1227 Ets Ets NNP 1228 1234 family family NN 1235 1241 member member NN 1242 1245 and and CC 1246 1254 NF-kappa NF-kappa NNP 1255 1256 B B NNP 1257 1263 family family NN 1264 1272 proteins protein NNS 1272 1273 . . . 1274 1279 These these DT 1280 1288 findings finding NNS 1289 1296 provide provide VBP 1297 1308 significant significant JJ 1309 1312 new new JJ 1313 1321 insights insight NNS 1322 1326 into into IN 1327 1330 the the DT 1331 1346 protein-protein protein-protein JJ 1347 1350 and and CC 1351 1362 protein-DNA protein-dna JJ 1363 1375 interactions interaction NNS 1376 1380 that that WDT 1381 1389 regulate regulate VBP 1390 1408 cell-type-specific cell-type-specific JJ 1409 1412 and and CC 1413 1422 inducible inducible JJ 1423 1428 IL-2R il-2r NN 1429 1434 alpha alpha NN 1435 1439 gene gene NN 1440 1450 expression expression NN 1451 1454 and and CC 1455 1459 also also RB 1460 1464 have have VBP 1465 1477 implications implication NNS 1478 1481 for for IN 1482 1487 other other JJ 1488 1493 genes gene NNS 1494 1503 regulated regulate VBN 1504 1506 by by IN 1507 1512 Elf-1 Elf-1 NNP 1513 1516 and and CC 1517 1525 NF-kappa NF-kappa NNP 1526 1527 B B NNP 1528 1534 family family NN 1535 1543 proteins protein NNS 1543 1544 . . .