0 10 Regulation regulation NN 11 13 of of IN 14 18 CD14 cd14 NN 19 29 expression expression NN 30 36 during during IN 37 46 monocytic monocytic JJ 47 62 differentiation differentiation NN 63 70 induced induce VBN 71 75 with with IN 76 77 1 1 CD 78 103 alpha,25-dihydroxyvitamin alpha,25-dihydroxyvitamin NN 104 106 D3 d3 NN 106 107 . . . 109 113 CD14 cd14 NN 113 114 , , , 115 116 a a DT 117 136 monocyte/macrophage monocyte/macrophage NN 137 145 receptor receptor NN 146 149 for for IN 150 153 the the DT 154 161 complex complex NN 162 164 of of IN 165 168 LPS LPS NNP 169 172 and and CC 173 176 LPS LPS NNP 177 184 binding binding NN 185 192 protein protein NN 192 193 , , , 194 196 is be VBZ 197 198 a a DT 199 214 differentiation differentiation NN 215 221 marker marker NN 222 225 for for IN 226 229 the the DT 230 249 monocyte/macrophage monocyte/macrophage NN 250 257 lineage lineage NN 257 258 . . . 259 261 We we PRP 262 266 have have VBP 267 275 analyzed analyze VBN 276 279 the the DT 280 290 regulation regulation NN 291 293 of of IN 294 298 CD14 cd14 NN 299 309 expression expression NN 310 316 during during IN 317 318 1 1 CD 319 344 alpha,25-dihydroxyvitamin alpha,25-dihydroxyvitamin NN 345 347 D3 D3 NNP 348 349 ( ( ( 349 363 VitD3)-induced vitd3)-induced JJ 364 373 monocytic monocytic JJ 374 389 differentiation differentiation NN 389 390 . . . 391 396 Using use VBG 397 401 FACS facs NN 401 402 , , , 403 411 Northern Northern NNP 412 420 blotting blotting NN 420 421 , , , 422 425 and and CC 426 433 nuclear nuclear JJ 434 440 run-on run-on JJ 441 449 analyses analysis NNS 449 450 , , , 451 453 we we PRP 454 465 demonstrate demonstrate VBP 466 470 that that IN 471 474 the the DT 475 488 up-regulation up-regulation NN 489 491 of of IN 492 496 CD14 cd14 NN 497 507 expression expression NN 508 514 during during IN 515 524 monocytic monocytic JJ 525 529 cell cell NN 530 540 maturation maturation NN 541 543 is be VBZ 544 553 regulated regulate VBN 554 560 mainly mainly RB 561 563 at at IN 564 567 the the DT 568 573 level level NN 574 576 of of IN 577 581 gene gene NN 582 595 transcription transcription NN 595 596 , , , 597 600 and and CC 601 605 that that IN 606 609 new new JJ 610 617 protein protein NN 618 627 synthesis synthesis NN 628 630 is be VBZ 631 639 required require VBN 640 643 for for IN 644 648 CD14 cd14 NN 649 658 induction induction NN 658 659 . . . 660 662 We we PRP 663 667 have have VBP 668 676 recently recently RB 677 683 cloned clone VBN 684 687 the the DT 688 692 CD14 cd14 NN 693 694 5 5 CD 694 695 ’ ' SYM 696 704 upstream upstream JJ 705 713 sequence sequence NN 714 717 and and CC 718 730 demonstrated demonstrate VBD 731 734 its its PRP$ 735 750 tissue-specific tissue-specific JJ 751 759 promoter promoter NN 760 768 activity activity NN 768 769 . . . 770 775 Using use VBG 776 782 stable stable JJ 783 795 transfection transfection NN 796 798 of of IN 799 802 the the DT 803 813 monocytoid monocytoid JJ 814 818 U937 u937 NN 819 823 cell cell NN 824 828 line line NN 829 833 with with IN 834 835 a a DT 836 842 series series NN 843 845 of of IN 846 854 deletion deletion NN 855 862 mutants mutant NNS 863 865 of of IN 866 869 the the DT 870 874 CD14 cd14 NN 875 876 5 5 CD 876 877 ’ ' SYM 878 886 upstream upstream JJ 887 895 sequence sequence NN 896 903 coupled couple VBN 904 906 to to TO 907 908 a a DT 909 917 reporter reporter NN 918 922 gene gene NN 923 932 construct construct NN 932 933 , , , 934 936 we we PRP 937 941 show show VBP 942 946 that that IN 947 949 bp bp NN 950 954 -128 -128 CD 955 957 to to TO 958 961 -70 -70 CD 962 964 is be VBZ 965 968 the the DT 969 977 critical critical JJ 978 984 region region NN 985 988 for for IN 989 992 the the DT 993 1002 induction induction NN 1003 1005 of of IN 1006 1010 CD14 cd14 NN 1011 1021 expression expression NN 1021 1022 . . . 1023 1027 This this DT 1028 1034 region region NN 1035 1043 contains contain VBZ 1044 1047 two two CD 1048 1055 binding bind VBG 1056 1061 sites site NNS 1062 1065 for for IN 1066 1069 the the DT 1070 1073 Sp1 sp1 NN 1074 1087 transcription transcription NN 1088 1094 factor factor NN 1094 1095 . . . 1096 1097 A a DT 1098 1102 3-bp 3-bp JJ 1103 1111 mutation mutation NN 1112 1114 at at IN 1115 1118 the the DT 1119 1125 distal distal JJ 1126 1137 Sp1-binding sp1-binding JJ 1138 1142 site site NN 1143 1146 not not RB 1147 1151 only only RB 1152 1162 eliminates eliminate VBZ 1163 1166 Sp1 sp1 NN 1167 1178 interaction interaction NN 1178 1179 , , , 1180 1183 but but CC 1184 1188 also also RB 1189 1198 abolishes abolish VBZ 1199 1203 most most JJS 1204 1206 of of IN 1207 1210 the the DT 1211 1216 VitD3 vitd3 NN 1217 1226 induction induction NN 1227 1229 of of IN 1230 1234 CD14 cd14 NN 1235 1245 expression expression NN 1245 1246 . . . 1247 1262 Electrophoretic electrophoretic JJ 1263 1271 mobility mobility NN 1272 1277 shift shift NN 1278 1286 analysis analysis NN 1287 1291 does do VBZ 1292 1295 not not RB 1296 1302 detect detect VB 1303 1304 a a DT 1305 1311 direct direct JJ 1312 1323 interaction interaction NN 1324 1326 of of IN 1327 1330 the the DT 1331 1335 CD14 cd14 NN 1336 1342 distal distal JJ 1343 1354 Sp1-binding sp1-binding JJ 1355 1359 site site NN 1360 1364 with with IN 1365 1368 the the DT 1369 1376 vitamin vitamin NN 1377 1379 D3 d3 NN 1380 1388 receptor receptor NN 1389 1392 and and CC 1393 1396 its its PRP$ 1397 1404 partner partner NN 1404 1405 , , , 1406 1409 the the DT 1410 1418 retinoid retinoid JJ 1419 1420 X x NN 1421 1429 receptor receptor NN 1429 1430 . . . 1431 1436 These these DT 1437 1441 data datum NNS 1442 1453 demonstrate demonstrate VBP 1454 1458 that that IN 1459 1464 VitD3 vitd3 NN 1465 1472 induces induce VBZ 1473 1477 CD14 cd14 NN 1478 1488 indirectly indirectly RB 1489 1496 through through IN 1497 1501 some some DT 1502 1514 intermediary intermediary JJ 1515 1521 factor factor NN 1521 1522 , , , 1523 1526 and and CC 1527 1534 suggest suggest VBP 1535 1536 a a DT 1537 1545 critical critical JJ 1546 1550 role role NN 1551 1554 for for IN 1555 1558 Sp1 sp1 NN 1559 1561 in in IN 1562 1566 this this DT 1567 1574 process process NN 1574 1575 . . .