0 10 Regulation regulation NN 11 13 of of IN 14 27 interleukin-1 interleukin-1 NN 28 32 beta beta NN 33 43 production production NN 44 46 by by IN 47 62 glucocorticoids glucocorticoid NNS 63 65 in in IN 66 71 human human JJ 72 81 monocytes monocyte NNS 81 82 : : : 83 86 the the DT 87 96 mechanism mechanism NN 97 99 of of IN 100 106 action action NN 107 114 depends depend VBZ 115 117 on on IN 118 121 the the DT 122 132 activation activation NN 133 139 signal signal NN 139 140 . . . 142 157 Glucocorticoids glucocorticoid NNS 158 161 are be VBP 162 167 known know VBN 168 170 to to TO 171 183 downregulate downregulate VB 184 197 interleukin-1 interleukin-1 NN 198 202 beta beta NN 203 213 production production NN 214 216 in in IN 217 226 monocytic monocytic JJ 227 232 cells cell NNS 233 235 by by IN 236 239 two two CD 240 249 different different JJ 250 259 mechanims mechanim NNS 259 260 : : : 261 267 direct direct JJ 268 278 inhibition inhibition NN 279 281 of of IN 282 285 the the DT 286 290 gene gene NN 291 304 transcription transcription NN 305 308 and and CC 309 324 destabilization destabilization NN 325 327 of of IN 328 331 the the DT 332 341 preformed preformed JJ 342 355 interleukin-1 interleukin-1 NN 356 360 beta beta NN 361 365 mRNA mrna NN 365 366 . . . 367 370 Now now RB 371 373 we we PRP 374 378 have have VBP 379 387 examined examine VBN 388 391 the the DT 392 398 effect effect NN 399 401 of of IN 402 405 the the DT 406 412 nature nature NN 413 415 of of IN 416 419 the the DT 420 428 monocyte monocyte NN 429 439 activating activate VBG 440 446 signal signal NN 447 449 on on IN 450 455 these these DT 456 459 two two CD 460 470 inhibitory inhibitory JJ 471 480 mechanims mechanim NNS 480 481 . . . 482 486 When when WRB 487 492 human human JJ 493 502 monocytes monocyte NNS 503 507 were be VBD 508 520 preincubated preincubate VBN 521 525 with with IN 526 539 dexamethasone dexamethasone NN 540 543 for for IN 544 545 1 1 CD 546 550 hour hour NN 551 554 and and CC 555 559 then then RB 560 570 stimulated stimulate VBN 571 577 either either CC 578 582 with with IN 583 592 bacterial bacterial JJ 593 611 lipopolysaccharide lipopolysaccharide NN 612 614 or or CC 615 622 phorbol phorbol NN 623 632 myristate myristate NN 632 633 , , , 634 636 it it PRP 637 640 was be VBD 641 646 found find VBN 647 651 that that IN 652 665 dexamethasone dexamethasone NN 666 675 inhibited inhibit VBD 676 679 the the DT 680 706 lipopolysaccharide-induced lipopolysaccharide-induced JJ 707 720 interleukin-1 interleukin-1 NN 721 725 beta beta NN 726 733 protein protein NN 734 744 production production NN 744 745 , , , 746 749 but but CC 750 753 the the DT 754 761 phorbol phorbol NN 762 779 myristate-induced myristate-induced JJ 780 790 production production NN 791 794 was be VBD 795 804 increased increase VBN 805 809 3-10 3-10 CD 810 814 fold fold RB 814 815 . . . 816 820 This this DT 821 831 difference difference NN 832 835 was be VBD 836 840 also also RB 841 845 seen see VBN 846 848 at at IN 849 852 the the DT 853 857 mRNA mrna NN 858 863 level level NN 863 864 . . . 865 869 When when WRB 870 883 dexamethasone dexamethasone NN 884 887 was be VBD 888 893 added add VBN 894 896 to to TO 897 900 the the DT 901 909 cultures culture NNS 910 911 3 3 CD 912 917 hours hour NNS 918 923 after after IN 924 927 the the DT 928 939 stimulators stimulator NNS 939 940 , , , 941 943 it it PRP 944 951 clearly clearly RB 952 961 decreased decrease VBD 962 965 the the DT 966 979 interleukin-1 interleukin-1 NN 980 984 beta beta NN 985 989 mRNA mrna NN 990 996 levels level NNS 997 1007 regardless regardless RB 1008 1010 of of IN 1011 1014 the the DT 1015 1025 stimulator stimulator NN 1026 1030 used use VBN 1031 1032 ( ( ( 1032 1040 although although IN 1041 1044 the the DT 1045 1051 effect effect NN 1052 1055 was be VBD 1056 1063 clearly clearly RB 1064 1070 weaker weak JJR 1071 1073 on on IN 1074 1077 the the DT 1078 1089 PMA-induced pma-induced JJ 1090 1094 mRNA mRNA NNP 1094 1095 ) ) ) 1095 1096 . . . 1097 1101 Thus thus RB 1102 1107 these these DT 1108 1112 data datum NNS 1113 1120 suggest suggest VBP 1121 1125 that that IN 1126 1129 the the DT 1130 1137 phorbol phorbol NN 1138 1155 myristate-induced myristate-induced JJ 1156 1162 signal signal NN 1163 1164 ( ( ( 1164 1173 prolonged prolong VBN 1174 1181 protein protein NN 1182 1188 kinase kinase NNP 1189 1190 C C NNP 1191 1201 activation activation NN 1201 1202 ? ? . 1202 1203 ) ) ) 1204 1210 cannot cannot MD 1211 1213 be be VB 1214 1223 inhibited inhibit VBN 1224 1226 by by IN 1227 1232 prior prior JJ 1233 1243 incubation incubation NN 1244 1248 with with IN 1249 1262 dexamethasone dexamethasone NN 1263 1266 and and CC 1267 1269 it it PRP 1270 1274 also also RB 1275 1283 protects protect VBZ 1284 1287 the the DT 1288 1295 induced induce VBN 1296 1300 mRNA mrna NN 1301 1304 for for IN 1305 1308 the the DT 1309 1320 degradative degradative JJ 1321 1327 action action NN 1328 1330 of of IN 1331 1344 dexamethasone dexamethasone NN 1344 1345 . . .