0 5 HIV-1 HIV-1 NNP 6 9 LTR LTR NNP 10 18 activity activity NN 19 21 in in IN 22 27 human human JJ 28 42 CD40-activated cd40-activated JJ 43 44 B B NNP 45 56 lymphocytes lymphocyte NNS 57 59 is be VBZ 60 69 dependent dependent JJ 70 72 on on IN 73 82 NF-kappaB NF-kappaB NNP 82 83 . . . 85 100 CD40-stimulated cd40-stimulated JJ 101 106 human human JJ 107 108 B B NNP 109 120 lymphocytes lymphocyte NNS 121 124 are be VBP 125 131 highly highly RB 132 142 permissive permissive JJ 143 145 to to TO 146 147 a a DT 148 158 productive productive JJ 159 168 infection infection NN 169 171 by by IN 172 175 the the DT 176 181 human human JJ 182 198 immunodeficiency immunodeficiency NN 199 204 virus virus NN 205 209 type type NN 210 211 1 1 CD 211 212 . . . 213 215 In in IN 216 221 these these DT 222 227 cells cell NNS 227 228 , , , 229 236 nuclear nuclear JJ 237 244 factors factor NNS 245 253 involved involve VBN 254 256 in in IN 257 267 activation activation NN 268 270 of of IN 271 274 the the DT 275 280 HIV-1 HIV-1 NNP 281 284 LTR LTR NNP 284 285 , , , 286 291 which which WDT 292 300 contains contain VBZ 301 304 the the DT 305 320 transcriptional transcriptional JJ 321 328 control control NN 329 337 elements element NNS 338 340 of of IN 341 344 the the DT 345 350 virus virus NN 350 351 , , , 352 355 are be VBP 356 363 unknown unknown JJ 363 364 . . . 365 374 Transient transient JJ 375 385 expression expression NN 386 392 assays assay NNS 393 397 with with IN 398 406 plasmids plasmid NNS 407 417 containing contain VBG 418 425 deleted delete VBN 426 431 parts part NNS 432 434 of of IN 435 438 the the DT 439 442 LTR LTR NNP 443 449 region region NN 450 456 linked link VBN 457 459 to to TO 460 461 a a DT 462 470 reporter reporter NN 471 475 gene gene NN 476 482 showed show VBD 483 487 that that IN 488 491 the the DT 492 501 NF-kappaB NF-kappaB NNP 502 509 binding binding NN 510 514 site site NN 515 518 was be VBD 519 528 essential essential JJ 529 532 for for IN 533 538 HIV-1 HIV-1 NNP 539 542 LTR LTR NNP 543 551 activity activity NN 552 554 in in IN 555 570 CD40-stimulated cd40-stimulated JJ 571 572 B B NNP 573 584 lymphocytes lymphocyte NNS 584 585 . . . 586 588 In in IN 589 597 addition addition NN 597 598 , , , 599 614 electrophoretic electrophoretic JJ 615 623 mobility mobility NN 624 629 shift shift NN 630 633 and and CC 634 644 supershift supershift NN 645 651 assays assay NNS 652 660 revealed reveal VBD 661 665 that that IN 666 675 important important JJ 676 685 NF-kappaB NF-kappaB NNP 686 693 binding binding NN 694 702 activity activity NN 703 711 composed compose VBN 712 714 of of IN 715 717 at at IN 718 723 least least JJS 724 727 p50 p50 NN 727 728 , , , 729 732 p65 p65 NN 732 733 , , , 734 737 and and CC 738 743 c-Rel c-rel NN 744 753 NF-kappaB NF-kappaB NNP 754 762 subunits subunit NNS 763 766 was be VBD 767 774 present present JJ 775 777 in in IN 778 784 nuclei nucleus NNS 785 787 of of IN 788 803 CD40-stimulated cd40-stimulated JJ 804 805 B B NNP 806 811 cells cell NNS 811 812 . . . 813 818 These these DT 819 826 results result NNS 827 834 confirm confirm VBP 835 837 at at IN 838 839 a a DT 840 849 molecular molecular JJ 850 855 level level NN 856 859 the the DT 860 867 ability ability NN 868 870 of of IN 871 876 HIV-1 HIV-1 NNP 877 879 to to TO 880 889 replicate replicate VB 890 892 in in IN 893 894 B B NNP 895 900 cells cell NNS 901 904 and and CC 905 909 that that IN 910 914 this this DT 915 923 activity activity NN 924 926 is be VBZ 927 935 strongly strongly RB 936 946 associated associate VBN 947 951 with with IN 952 961 NF-kappaB NF-kappaB NNP 961 962 . . .