0 12 Differential differential JJ 13 23 regulation regulation NN 24 26 of of IN 27 31 IL-6 IL-6 NNP 32 36 gene gene NN 37 50 transcription transcription NN 51 54 and and CC 55 65 expression expression NN 66 68 by by IN 69 73 IL-4 il-4 NN 74 77 and and CC 78 83 IL-10 il-10 NN 84 86 in in IN 87 92 human human JJ 93 102 monocytic monocytic JJ 103 107 cell cell NN 108 113 lines line NNS 113 114 . . . 116 120 IL-4 il-4 NN 121 124 and and CC 125 130 IL-10 il-10 NN 131 138 inhibit inhibit VBP 139 142 the the DT 143 151 cytokine cytokine NN 152 162 production production NN 163 166 and and CC 167 171 mRNA mrna NN 172 182 expression expression NN 183 185 by by IN 186 207 monocytes/macrophages monocytes/macrophage NNS 207 208 . . . 209 211 To to TO 212 223 investigate investigate VB 224 227 the the DT 228 237 molecular molecular JJ 238 247 mechanism mechanism NN 248 250 of of IN 251 254 the the DT 255 265 inhibitory inhibitory JJ 266 272 effect effect NN 273 275 on on IN 276 291 transcriptional transcriptional JJ 292 294 or or CC 295 315 post-transcriptional post-transcriptional JJ 316 326 regulation regulation NN 327 329 of of IN 330 334 IL-6 IL-6 NNP 335 339 gene gene NN 340 350 expression expression NN 351 353 by by IN 354 358 IL-4 il-4 NN 359 362 and and CC 363 368 IL-10 il-10 NN 368 369 , , , 370 372 we we PRP 373 380 studied study VBD 381 385 IL-6 il-6 NN 386 396 production production NN 396 397 , , , 398 408 expression expression NN 409 414 level level NN 415 417 of of IN 418 422 IL-6 IL-6 NNP 423 427 mRNA mRNA NNP 427 428 , , , 429 433 IL-6 IL-6 NNP 434 442 promoter promoter NN 443 451 activity activity NN 451 452 , , , 453 468 transcriptional transcriptional JJ 469 477 activity activity NN 478 480 of of IN 481 490 NF-kappaB NF-kappaB NNP 491 494 and and CC 495 502 NF-IL-6 NF-IL-6 NNP 502 503 , , , 504 507 and and CC 508 512 IL-6 IL-6 NNP 513 517 mRNA mrna NN 518 527 stability stability NN 528 530 in in IN 531 536 human human JJ 537 546 monocytic monocytic JJ 547 551 cell cell NN 552 557 lines line NNS 557 558 , , , 559 564 THP-1 thp-1 NN 565 568 and and CC 569 573 U937 u937 NN 573 574 , , , 575 585 stimulated stimulate VBN 586 588 by by IN 589 592 PMA PMA NNP 593 596 and and CC 597 600 LPS LPS NNP 601 603 in in IN 604 607 the the DT 608 615 absence absence NN 616 618 or or CC 619 622 the the DT 623 631 presence presence NN 632 634 of of IN 635 639 IL-4 il-4 NN 640 642 or or CC 643 648 IL-10 il-10 NN 648 649 . . . 650 654 Both both CC 655 659 IL-4 il-4 NN 660 663 and and CC 664 669 IL-10 il-10 NN 670 674 were be VBD 675 679 seen see VBN 680 682 to to TO 683 690 inhibit inhibit VB 691 695 IL-6 il-6 NN 696 706 production production NN 707 710 and and CC 711 714 the the DT 715 725 expression expression NN 726 728 of of IN 729 733 IL-6 IL-6 NNP 734 738 mRNA mRNA NNP 739 741 in in IN 742 746 both both CC 747 756 monocytic monocytic JJ 757 761 cell cell NN 762 767 lines line NNS 768 775 studied study VBN 775 776 . . . 777 779 In in IN 780 795 chloramphenicol chloramphenicol NN 796 813 acetyltransferase acetyltransferase NN 814 820 assays assay NNS 820 821 , , , 822 831 utilizing utilize VBG 832 835 the the DT 836 845 transient transient JJ 846 858 transfection transfection NN 859 861 of of IN 862 863 a a DT 864 879 chloramphenicol chloramphenicol NN 880 897 acetyltransferase acetyltransferase NN 898 906 reporter reporter NN 907 914 plasmid plasmid NN 915 925 containing contain VBG 926 929 the the DT 930 934 IL-6 il-6 NN 935 939 gene gene NN 940 948 promoter promoter NN 948 949 , , , 950 954 IL-4 il-4 NN 954 955 , , , 956 959 but but CC 960 963 not not RB 964 969 IL-10 IL-10 NNP 969 970 , , , 971 981 suppressed suppress VBD 982 985 the the DT 986 1001 transcriptional transcriptional JJ 1002 1010 activity activity NN 1011 1013 of of IN 1014 1017 the the DT 1018 1022 IL-6 IL-6 NNP 1023 1027 gene gene NN 1028 1036 promoter promoter NN 1037 1047 stimulated stimulate VBN 1048 1050 by by IN 1051 1054 PMA PMA NNP 1055 1058 and and CC 1059 1062 LPS LPS NNP 1062 1063 . . . 1064 1079 Electrophoretic electrophoretic JJ 1080 1088 mobility mobility NN 1089 1094 shift shift NN 1095 1101 assays assay NNS 1102 1108 showed show VBD 1109 1113 that that IN 1114 1118 IL-4 il-4 NN 1118 1119 , , , 1120 1123 but but CC 1124 1127 not not RB 1128 1133 IL-10 IL-10 NNP 1133 1134 , , , 1135 1144 inhibited inhibit VBD 1145 1152 nuclear nuclear JJ 1153 1162 NF-kappaB NF-kappaB NNP 1163 1171 activity activity NN 1171 1172 , , , 1173 1176 and and CC 1177 1181 that that IN 1182 1186 IL-4 il-4 NN 1187 1190 and and CC 1191 1196 IL-10 il-10 NN 1197 1200 did do VBD 1201 1204 not not RB 1205 1211 affect affect VB 1212 1219 NF-IL-6 nf-il-6 NN 1220 1228 activity activity NN 1228 1229 . . . 1230 1232 On on IN 1233 1236 the the DT 1237 1242 other other JJ 1243 1247 hand hand NN 1247 1248 , , , 1249 1254 IL-10 IL-10 NNP 1255 1263 enhanced enhance VBD 1264 1267 the the DT 1268 1279 degradation degradation NN 1280 1282 of of IN 1283 1287 IL-6 IL-6 NNP 1288 1292 mRNA mRNA NNP 1293 1295 in in IN 1296 1297 a a DT 1298 1302 mRNA mrna NN 1303 1312 stability stability NN 1313 1318 assay assay NN 1318 1319 . . . 1320 1325 These these DT 1326 1333 results result NNS 1334 1341 suggest suggest VBP 1342 1346 that that IN 1347 1351 IL-4 il-4 NN 1352 1355 may may MD 1356 1363 inhibit inhibit VB 1364 1367 the the DT 1368 1381 transcription transcription NN 1382 1384 of of IN 1385 1388 the the DT 1389 1393 IL-6 il-6 NN 1394 1398 gene gene NN 1399 1401 by by IN 1402 1411 affecting affect VBG 1412 1421 NF-kappaB NF-kappaB NNP 1422 1429 binding binding NN 1430 1438 activity activity NN 1438 1439 , , , 1440 1445 while while IN 1446 1451 IL-10 IL-10 NNP 1452 1455 may may MD 1456 1463 inhibit inhibit VB 1464 1467 the the DT 1468 1472 IL-6 IL-6 NNP 1473 1477 mRNA mrna NN 1478 1484 levels level NNS 1485 1507 post-transcriptionally post-transcriptionally RB 1507 1508 , , , 1509 1516 without without IN 1517 1528 suppressing suppress VBG 1529 1537 promoter promoter NN 1538 1546 activity activity NN 1546 1547 . . . 1548 1557 Therefore therefore RB 1557 1558 , , , 1559 1561 we we PRP 1562 1570 conclude conclude VBP 1571 1575 that that IN 1576 1580 IL-4 il-4 NN 1581 1584 and and CC 1585 1590 IL-10 il-10 NN 1591 1598 inhibit inhibit VBP 1599 1603 IL-6 il-6 NN 1604 1614 production production NN 1615 1617 by by IN 1618 1627 different different JJ 1628 1638 mechanisms mechanism NNS 1639 1641 in in IN 1642 1647 human human JJ 1648 1657 monocytic monocytic JJ 1658 1662 cell cell NN 1663 1668 lines line NNS 1668 1669 . . .