0 3 rel rel NN 4 6 Is be VBZ 7 14 rapidly rapidly RB 15 38 tyrosine-phosphorylated tyrosine-phosphorylated JJ 39 48 following follow VBG 49 67 granulocyte-colony granulocyte-colony JJ 68 79 stimulating stimulating NN 80 86 factor factor NN 87 96 treatment treatment NN 97 99 of of IN 100 105 human human JJ 106 117 neutrophils neutrophil NNS 117 118 . . . 120 131 Stimulation stimulation NN 132 134 of of IN 135 146 neutrophils neutrophil NNS 147 151 with with IN 152 170 granulocyte-colony granulocyte-colony JJ 171 182 stimulating stimulating NN 183 189 factor factor NN 190 191 ( ( ( 191 196 G-CSF G-CSF NNP 196 197 ) ) ) 198 205 results result VBZ 206 208 in in IN 209 211 an an DT 212 220 enhanced enhance VBN 221 232 respiratory respiratory JJ 233 238 burst burst NN 238 239 , , , 240 249 prolonged prolong VBN 250 258 survival survival NN 258 259 , , , 260 263 and and CC 264 273 increased increase VBN 274 279 tumor tumor NN 280 284 cell cell NN 285 292 killing killing NN 292 293 . . . 294 297 The the DT 298 305 effects effect NNS 306 308 of of IN 309 314 G-CSF G-CSF NNP 315 318 are be VBP 319 327 mediated mediate VBN 328 330 by by IN 331 338 binding bind VBG 339 341 to to TO 342 350 specific specific JJ 350 351 , , , 352 356 high high JJ 357 365 affinity affinity NN 366 375 receptors receptor NNS 375 376 . . . 377 382 G-CSF G-CSF NNP 383 392 receptors receptor NNS 393 397 lack lack VBP 398 407 intrinsic intrinsic JJ 408 416 tyrosine tyrosine NN 417 423 kinase kinase NNP 424 432 activity activity NN 432 433 , , , 434 437 but but CC 438 448 activation activation NN 449 451 of of IN 452 455 the the DT 456 464 receptor receptor NN 465 472 results result VBZ 473 475 in in IN 476 479 the the DT 480 485 rapid rapid JJ 486 495 induction induction NN 496 498 of of IN 499 507 tyrosine tyrosine NN 508 514 kinase kinase NNP 515 523 activity activity NN 523 524 . . . 525 544 Antiphosphotyrosine Antiphosphotyrosine NNP 545 556 immunoblots immunoblot NNS 557 559 of of IN 560 565 whole whole JJ 566 570 cell cell NN 571 578 lysates lysate NNS 579 587 prepared prepare VBN 588 592 from from IN 593 604 neutrophils neutrophil NNS 605 609 show show VBP 610 614 that that IN 615 618 the the DT 619 624 G-CSF G-CSF NNP 625 632 rapidly rapidly RB 633 640 induces induce VBZ 641 650 prominent prominent JJ 651 659 tyrosine tyrosine NN 660 675 phosphorylation phosphorylation NN 676 678 of of IN 679 680 a a DT 681 688 protein protein NN 689 691 of of IN 692 693 a a DT 694 702 relative relative JJ 703 712 molecular molecular JJ 713 717 mass mass NN 718 720 of of IN 721 723 80 80 CD 724 727 kDa kda NN 727 728 . . . 729 734 Using use VBG 735 747 monospecific monospecific JJ 748 758 antibodies antibody NNS 758 759 , , , 760 763 the the DT 764 770 80-kDa 80-kda JJ 771 794 tyrosine-phosphorylated tyrosine-phosphorylated JJ 795 802 protein protein NN 803 806 has have VBZ 807 811 been be VBN 812 817 shown show VBN 818 820 to to TO 821 823 be be VB 824 832 p80c-rel p80c-rel NN 832 833 , , , 834 835 a a DT 836 850 proto-oncogene proto-oncogene NN 851 860 belonging belong VBG 861 863 to to TO 864 865 a a DT 866 872 family family NN 873 875 of of IN 876 891 transcriptional transcriptional JJ 892 902 regulators regulator NNS 903 908 which which WDT 909 916 include include VBP 917 922 NF-kB NF-kB NNP 922 923 . . . 924 927 The the DT 928 937 induction induction NN 938 940 of of IN 941 949 tyrosine tyrosine NN 950 965 phosphorylation phosphorylation NN 966 968 of of IN 969 977 p80c-rel p80c-rel NN 978 981 was be VBD 982 988 unique unique JJ 989 991 to to TO 992 997 G-CSF G-CSF NNP 998 1000 in in IN 1001 1005 that that DT 1006 1028 granulocyte-macrophage granulocyte-macrophage JJ 1029 1035 colony colony NN 1036 1047 stimulating stimulating NN 1048 1054 factor factor NN 1055 1060 which which WDT 1061 1065 also also RB 1066 1076 stimulates stimulate VBZ 1077 1088 neutrophils neutrophil NNS 1089 1092 and and CC 1093 1100 induces induce VBZ 1101 1109 tyrosine tyrosine NN 1110 1125 phosphorylation phosphorylation NN 1126 1130 does do VBZ 1131 1134 not not RB 1135 1141 result result VB 1142 1144 in in IN 1145 1153 tyrosine tyrosine NN 1154 1169 phosphorylation phosphorylation NN 1170 1172 of of IN 1173 1181 p80c-rel p80c-rel NN 1181 1182 . . . 1183 1186 The the DT 1187 1199 consequences consequence NNS 1200 1202 of of IN 1203 1211 p80c-rel p80c-rel NN 1212 1220 tyrosine tyrosine NN 1221 1236 phosphorylation phosphorylation NN 1237 1240 are be VBP 1241 1244 not not RB 1245 1248 yet yet RB 1249 1254 known know VBN 1254 1255 ; ; : 1256 1263 however however RB 1263 1264 , , , 1265 1288 tyrosine-phosphorylated tyrosine-phosphorylated JJ 1289 1297 p80c-rel p80c-rel NN 1298 1300 is be VBZ 1301 1308 capable capable JJ 1309 1311 of of IN 1312 1319 binding bind VBG 1320 1322 to to TO 1323 1326 DNA DNA NNP 1326 1327 , , , 1328 1331 and and CC 1332 1337 G-CSF G-CSF NNP 1338 1349 stimulation stimulation NN 1350 1357 results result VBZ 1358 1360 in in IN 1361 1363 an an DT 1364 1372 increase increase NN 1373 1375 in in IN 1376 1379 the the DT 1380 1386 amount amount NN 1387 1389 of of IN 1390 1398 p80c-rel p80c-rel NN 1399 1404 which which WDT 1405 1410 binds bind VBZ 1411 1413 to to TO 1414 1417 DNA DNA NNP 1417 1418 . . . 1419 1424 These these DT 1425 1432 results result NNS 1433 1444 demonstrate demonstrate VBP 1445 1449 that that IN 1450 1453 one one CD 1454 1456 of of IN 1457 1460 the the DT 1461 1466 first first JJ 1467 1478 biochemical biochemical JJ 1479 1485 events event NNS 1486 1491 which which WDT 1492 1498 occurs occur VBZ 1499 1501 in in IN 1502 1513 neutrophils neutrophil NNS 1514 1523 following follow VBG 1524 1529 G-CSF G-CSF NNP 1530 1541 stimulation stimulation NN 1541 1542 , , , 1543 1553 activation activation NN 1554 1556 of of IN 1557 1558 a a DT 1559 1567 tyrosine tyrosine NN 1568 1574 kinase kinase NNP 1574 1575 , , , 1576 1581 leads lead VBZ 1582 1590 directly directly RB 1591 1593 to to TO 1594 1597 the the DT 1598 1606 tyrosine tyrosine NN 1607 1622 phosphorylation phosphorylation NN 1623 1625 of of IN 1626 1634 p80c-rel p80c-rel NN 1634 1635 . . . 1636 1640 Thus thus RB 1640 1641 , , , 1642 1645 the the DT 1646 1654 tyrosine tyrosine NN 1655 1661 kinase kinase NNP 1662 1671 activated activate VBN 1672 1674 by by IN 1675 1680 G-CSF G-CSF NNP 1681 1688 appears appear VBZ 1689 1691 to to TO 1692 1700 directly directly RB 1701 1710 transduce transduce VB 1711 1712 a a DT 1713 1719 signal signal NN 1720 1722 to to TO 1723 1724 a a DT 1725 1732 protein protein NN 1733 1738 which which WDT 1739 1748 functions function VBZ 1749 1751 as as IN 1752 1753 a a DT 1754 1769 transcriptional transcriptional JJ 1770 1779 regulator regulator NN 1779 1780 . . .