0 3 The the DT 4 20 56-59-kilodalton 56-59-kilodalton JJ 21 28 protein protein NN 29 39 identified identify VBN 40 42 in in IN 43 56 untransformed untransformed JJ 57 64 steroid steroid NN 65 73 receptor receptor NN 74 83 complexes complex NNS 84 86 is be VBZ 87 88 a a DT 89 95 unique unique JJ 96 103 protein protein NN 104 108 that that WDT 109 115 exists exist VBZ 116 118 in in IN 119 126 cytosol cytosol NN 127 129 in in IN 130 131 a a DT 132 139 complex complex NN 140 144 with with IN 145 149 both both CC 150 153 the the DT 154 157 70- 70- JJ 158 161 and and CC 162 175 90-kilodalton 90-kilodalton JJ 176 180 heat heat NN 181 186 shock shock NN 187 195 proteins protein NNS 195 196 . . . 198 200 It it PRP 201 204 has have VBZ 205 215 previously previously RB 216 220 been be VBN 221 226 shown show VBN 227 231 that that IN 232 234 9S 9s NN 234 235 , , , 236 249 untransformed untransformed JJ 250 259 progestin progestin NN 259 260 , , , 261 269 estrogen estrogen NN 269 270 , , , 271 279 androgen androgen NN 279 280 , , , 281 284 and and CC 285 299 glucocorticoid glucocorticoid NN 300 308 receptor receptor NN 309 318 complexes complex NNS 319 321 in in IN 322 328 rabbit rabbit NN 329 336 uterine uterine NN 337 340 and and CC 341 346 liver liver NN 347 355 cytosols cytosol NNS 356 363 contain contain VBP 364 365 a a DT 366 372 59-kDa 59-kda JJ 373 380 protein protein NN 381 382 [ [ ( 382 385 Tai Tai NNP 385 386 , , , 387 391 P.K. P.K. NNP 391 392 , , , 393 398 Maeda Maeda NNP 398 399 , , , 400 402 Y. Y. NNP 402 403 , , , 404 409 Nakao Nakao NNP 409 410 , , , 411 413 K. K. NNP 413 414 , , , 415 420 Wakim Wakim NNP 420 421 , , , 422 426 N.G. N.G. NNP 426 427 , , , 428 435 Duhring Duhring NNP 435 436 , , , 437 441 J.L. J.L. NNP 441 442 , , , 443 444 & & CC 445 450 Faber Faber NNP 450 451 , , , 452 462 L.E.(1986) L.E.(1986) NNP 463 475 Biochemistry Biochemistry NNP 476 478 25 25 CD 478 479 , , , 480 489 5269-5275 5269-5275 CD 489 490 ] ] ) 490 491 . . . 492 494 In in IN 495 499 this this DT 500 504 work work NN 505 507 we we PRP 508 512 show show VBP 513 517 that that IN 518 521 the the DT 522 532 monoclonal monoclonal JJ 533 541 antibody antibody NN 542 544 KN KN NNP 545 552 382/EC1 382/EC1 NNP 553 559 raised raise VBN 560 567 against against IN 568 571 the the DT 572 578 rabbit rabbit NN 579 585 59-kDa 59-kda JJ 586 593 protein protein NN 594 600 reacts react VBZ 601 605 with with IN 606 608 9S 9s NN 608 609 , , , 610 623 untransformed untransformed JJ 624 638 glucocorticoid glucocorticoid NN 639 647 receptor receptor NN 648 657 complexes complex NNS 658 660 in in IN 661 668 cytosol cytosol NN 669 677 prepared prepare VBN 678 682 from from IN 683 688 human human JJ 689 693 IM-9 IM-9 NNP 694 705 lymphocytes lymphocyte NNS 706 709 but but CC 710 713 not not RB 714 718 with with IN 719 721 4S 4s NN 722 738 salt-transformed salt-transformed JJ 739 748 receptors receptor NNS 748 749 . . . 750 753 The the DT 754 759 human human JJ 760 767 protein protein NN 768 778 recognized recognize VBN 779 781 by by IN 782 785 the the DT 786 789 EC1 ec1 NN 790 798 antibody antibody NN 799 801 is be VBZ 802 803 a a DT 804 810 56-kDa 56-kda JJ 811 818 protein protein NN 819 820 ( ( ( 820 823 p56 p56 NN 823 824 ) ) ) 825 827 of of IN 828 836 moderate moderate JJ 837 846 abundance abundance NN 847 854 located locate VBN 855 868 predominantly predominantly RB 869 871 in in IN 872 875 the the DT 876 885 cytoplasm cytoplasm NN 886 888 by by IN 889 897 indirect indirect JJ 898 916 immunofluorescence immunofluorescence NN 916 917 . . . 918 923 There there EX 924 927 are be VBP 928 930 at at IN 931 936 least least JJS 937 940 six six CD 941 950 isomorphs isomorph NNS 951 953 of of IN 954 957 p56 p56 NN 958 960 by by IN 961 976 two-dimensional two-dimensional JJ 977 980 gel gel NN 981 989 analysis analysis NN 989 990 . . . 991 1001 N-Terminal n-terminal JJ 1002 1012 sequencing sequencing NN 1013 1014 ( ( ( 1014 1016 20 20 CD 1017 1022 amino amino JJ 1023 1028 acids acid NNS 1028 1029 ) ) ) 1030 1035 shows show VBZ 1036 1040 that that IN 1041 1044 p56 p56 NN 1045 1047 is be VBZ 1048 1049 a a DT 1050 1056 unique unique JJ 1057 1062 human human JJ 1063 1070 protein protein NN 1070 1071 . . . 1072 1076 When when WRB 1077 1080 p56 p56 NN 1081 1083 is be VBZ 1084 1098 immunoadsorbed immunoadsorbe VBN 1099 1103 from from IN 1104 1108 IM-9 IM-9 NNP 1109 1113 cell cell NN 1114 1121 cytosol cytosol NN 1121 1122 , , , 1123 1127 both both CC 1128 1131 the the DT 1132 1135 70- 70- JJ 1136 1139 and and CC 1140 1146 90-kDa 90-kda JJ 1147 1151 heat heat NN 1152 1157 shock shock NN 1158 1166 proteins protein NNS 1167 1170 are be VBP 1171 1181 coadsorbed coadsorbe VBN 1182 1184 in in IN 1185 1187 an an DT 1188 1203 immune-specific immune-specific JJ 1204 1210 manner manner NN 1210 1211 . . . 1212 1219 Neither neither CC 1220 1224 heat heat NN 1225 1230 shock shock NN 1231 1238 protein protein NN 1239 1245 reacts react VBZ 1246 1254 directly directly RB 1255 1259 with with IN 1260 1263 the the DT 1264 1267 EC1 ec1 NN 1268 1276 antibody antibody NN 1276 1277 . . . 1278 1280 We we PRP 1281 1289 conclude conclude VBP 1290 1294 that that IN 1295 1298 p56 p56 NN 1299 1305 exists exist VBZ 1306 1308 in in IN 1309 1316 cytosol cytosol NN 1317 1319 in in IN 1320 1321 a a DT 1322 1328 higher high JJR 1329 1334 order order NN 1335 1342 complex complex NN 1343 1353 containing contain VBG 1354 1359 hsp70 hsp70 NN 1360 1363 and and CC 1364 1369 hsp90 hsp90 NN 1369 1370 , , , 1371 1375 both both DT 1376 1378 of of IN 1379 1384 which which WDT 1385 1387 in in IN 1388 1392 turn turn NN 1393 1397 have have VBP 1398 1402 been be VBN 1403 1408 found find VBN 1409 1411 to to TO 1412 1414 be be VB 1415 1425 associated associate VBN 1426 1430 with with IN 1431 1444 untransformed untransformed JJ 1445 1452 steroid steroid NN 1453 1462 receptors receptor NNS 1462 1463 . . .