0 7 Protein protein NN 8 14 kinase kinase NNP 15 24 inhibitor inhibitor NN 25 28 H-7 h-7 NN 29 35 blocks block VBZ 36 48 accumulation accumulation NN 49 51 of of IN 52 61 unspliced unspliced JJ 62 66 mRNA mrna NN 67 69 of of IN 70 75 human human JJ 76 82 T-cell t-cell NN 83 91 leukemia leukemia NN 92 97 virus virus NN 98 102 type type NN 103 104 I i CD 105 106 ( ( ( 106 112 HTLV-I HTLV-I NNP 112 113 ) ) ) 113 114 . . . 116 119 Rex Rex NNP 119 120 , , , 121 124 the the DT 125 145 post-transcriptional post-transcriptional JJ 146 155 regulator regulator NN 156 158 of of IN 159 164 human human JJ 165 171 T-cell t-cell NN 172 180 leukemia leukemia NN 181 186 virus virus NN 187 191 type type NN 192 193 I i CD 194 195 ( ( ( 195 201 HTLV-I HTLV-I NNP 201 202 ) ) ) 202 203 , , , 204 206 is be VBZ 207 212 known know VBN 213 215 to to TO 216 222 induce induce VB 223 235 accumulation accumulation NN 236 238 of of IN 239 242 the the DT 243 252 unspliced unspliced JJ 253 258 viral viral JJ 259 266 gag-pol gag-pol NN 267 271 mRNA mrna NN 271 272 . . . 273 276 Rex Rex NNP 277 279 is be VBZ 280 281 a a DT 282 296 phosphoprotein phosphoprotein NN 297 302 found find VBN 303 305 in in IN 306 309 the the DT 310 314 cell cell NN 315 324 nucleolus nucleolus NN 324 325 , , , 326 331 whose whose WP$ 332 340 function function NN 341 344 may may MD 345 347 be be VB 348 357 regulated regulate VBN 358 360 by by IN 361 364 its its PRP$ 365 377 localization localization NN 378 381 and and CC 382 397 phosphorylation phosphorylation NN 397 398 . . . 399 401 We we PRP 402 406 have have VBP 407 415 examined examine VBN 416 419 the the DT 420 424 role role NN 425 427 of of IN 428 443 phosphorylation phosphorylation NN 444 446 on on IN 447 450 Rex Rex NNP 451 459 function function NN 460 462 by by IN 463 468 using use VBG 469 470 a a DT 471 478 protein protein NN 479 485 kinase kinase NNP 486 495 inhibitor inhibitor NN 495 496 , , , 497 500 H-7 H-7 NNP 501 502 [ [ ( 502 549 1-(5-isoquinolinyl-sulfonyl)-2-methylpiperazine 1-(5-isoquinolinyl-sulfonyl)-2-methylpiperazine NN 549 550 ] ] ) 550 551 . . . 552 561 Treatment Treatment NNP 562 564 of of IN 565 567 an an DT 568 574 HTLV-I HTLV-I NNP 575 583 infected infected JJ 584 589 human human JJ 590 596 T-cell t-cell NN 597 601 line line NN 602 606 with with IN 607 610 H-7 H-7 NNP 611 618 blocked block VBD 619 631 specifically specifically RB 632 644 accumulation accumulation NN 645 647 of of IN 648 651 the the DT 652 661 unspliced unspliced JJ 662 669 gag-pol gag-pol NN 670 674 mRNA mrna NN 674 675 , , , 676 685 resulting result VBG 686 688 in in IN 689 692 the the DT 693 702 decreased decrease VBN 703 706 Gag gag NN 707 714 protein protein NN 715 724 synthesis synthesis NN 725 729 that that WDT 730 741 corresponds correspond VBZ 742 746 with with IN 747 750 the the DT 751 760 decreased decrease VBN 761 763 in in FW 764 768 vivo vivo FW 769 784 phosphorylation phosphorylation NN 785 787 of of IN 788 791 Rex Rex NNP 791 792 . . . 793 795 In in IN 796 804 contrast contrast NN 804 805 , , , 806 811 other other JJ 812 817 viral viral JJ 818 821 and and CC 822 830 cellular cellular JJ 831 839 products product NNS 840 844 have have VBP 845 848 not not RB 849 853 been be VBN 854 864 influenced influence VBN 865 867 by by IN 868 871 the the DT 872 877 level level NN 878 880 of of IN 881 884 H-7 H-7 NNP 885 889 used use VBN 889 890 . . . 891 900 Therefore therefore RB 900 901 , , , 902 905 the the DT 906 921 phosphorylation phosphorylation NN 922 924 of of IN 925 928 Rex Rex NNP 929 931 is be VBZ 932 940 required require VBN 941 944 for for IN 945 948 the the DT 949 954 viral viral JJ 955 958 RNA RNA NNP 959 968 partition partition NN 969 971 of of IN 972 978 HTLV-I HTLV-I NNP 978 979 . . .