0 17 Calcium-dependent calcium-dependent JJ 18 33 immediate-early immediate-early JJ 34 38 gene gene NN 39 48 induction induction NN 49 51 in in IN 52 63 lymphocytes lymphocyte NNS 64 66 is be VBZ 67 77 negatively negatively RB 78 87 regulated regulate VBN 88 90 by by IN 91 100 p21Ha-ras p21ha-ras NN 100 101 . . . 103 106 The the DT 107 116 induction induction NN 117 119 of of IN 120 135 immediate-early immediate-early JJ 136 137 ( ( ( 137 139 IE ie JJ 139 140 ) ) ) 141 149 response response NN 150 155 genes gene NNS 155 156 , , , 157 161 such such JJ 162 164 as as IN 165 170 egr-1 egr-1 NN 170 171 , , , 172 177 c-fos c-fos NN 177 178 , , , 179 182 and and CC 183 188 c-jun c-jun NN 188 189 , , , 190 196 occurs occur VBZ 197 204 rapidly rapidly RB 205 210 after after IN 211 214 the the DT 215 225 activation activation NN 226 228 of of IN 229 230 T t NN 231 242 lymphocytes lymphocyte NNS 242 243 . . . 244 247 The the DT 248 255 process process NN 256 258 of of IN 259 269 activation activation NN 270 278 involves involve VBZ 279 286 calcium calcium NN 287 299 mobilization mobilization NN 299 300 , , , 301 311 activation activation NN 312 314 of of IN 315 322 protein protein NN 323 329 kinase kinase NNP 330 331 C C NNP 332 333 ( ( ( 333 336 PKC PKC NNP 336 337 ) ) ) 337 338 , , , 339 342 and and CC 343 358 phosphorylation phosphorylation NN 359 361 of of IN 362 370 tyrosine tyrosine NN 371 379 kinases. kinases. NN 380 388 p21(ras) p21(ras) NN 388 389 , , , 390 391 a a DT 392 399 guanine guanine NN 400 410 nucleotide nucleotide NN 411 418 binding binding NN 419 425 factor factor NN 425 426 , , , 427 435 mediates mediate VBZ 436 442 T-cell t-cell NN 443 449 signal signal NN 450 462 transduction transduction NN 463 470 through through IN 471 484 PKC-dependent pkc-dependent JJ 485 488 and and CC 489 504 PKC-independent pkc-independent JJ 505 513 pathways pathway NNS 513 514 . . . 515 518 The the DT 519 530 involvement involvement NN 531 533 of of IN 534 542 p21(ras) p21(ras) NN 543 545 in in IN 546 549 the the DT 550 560 regulation regulation NN 561 563 of of IN 564 581 calcium-dependent calcium-dependent JJ 582 589 signals signal NNS 590 593 has have VBZ 594 598 been be VBN 599 608 suggested suggest VBN 609 616 through through IN 617 625 analysis analysis NN 626 628 of of IN 629 632 its its PRP$ 633 637 role role NN 638 640 in in IN 641 644 the the DT 645 655 activation activation NN 656 658 of of IN 659 664 NF-AT NF-AT NNP 664 665 . . . 666 668 We we PRP 669 673 have have VBP 674 686 investigated investigate VBN 687 690 the the DT 691 701 inductions induction NNS 702 704 of of IN 705 708 the the DT 709 711 IE ie JJ 712 717 genes gene NNS 718 720 in in IN 721 729 response response NN 730 732 to to TO 733 740 calcium calcium NN 741 748 signals signal NNS 749 751 in in IN 752 758 Jurkat Jurkat NNP 759 764 cells cell NNS 765 766 ( ( ( 766 768 in in IN 769 772 the the DT 773 781 presence presence NN 782 784 of of IN 785 794 activated activate VBN 795 804 p21(ras)) p21(ras)) NN 805 808 and and CC 809 814 their their PRP$ 815 825 correlated correlate VBN 826 838 consequences consequence NNS 838 839 . . . 840 843 The the DT 844 854 expression expression NN 855 857 of of IN 858 867 activated activate VBN 868 876 p21(ras) p21(ras) NN 877 887 negatively negatively RB 888 897 regulated regulate VBD 898 901 the the DT 902 911 induction induction NN 912 914 of of IN 915 917 IE ie JJ 918 923 genes gene NNS 924 926 by by IN 927 934 calcium calcium NN 935 944 ionophore ionophore NN 944 945 . . . 946 950 This this DT 951 961 inhibition inhibition NN 962 964 of of IN 965 982 calcium-activated calcium-activated JJ 983 985 IE IE NNP 986 990 gene gene NN 991 1000 induction induction NN 1001 1004 was be VBD 1005 1013 reversed reverse VBN 1014 1016 by by IN 1017 1026 treatment treatment NN 1027 1031 with with IN 1032 1043 cyclosporin cyclosporin NN 1044 1045 A a NN 1045 1046 , , , 1047 1057 suggesting suggest VBG 1058 1061 the the DT 1062 1073 involvement involvement NN 1074 1076 of of IN 1077 1088 calcineurin calcineurin NN 1089 1091 in in IN 1092 1096 this this DT 1097 1107 regulation regulation NN 1107 1108 . . . 1109 1110 A a DT 1111 1116 later later JJ 1117 1123 result result NN 1124 1126 of of IN 1127 1137 inhibition inhibition NN 1138 1140 of of IN 1141 1145 this this DT 1146 1156 activation activation NN 1157 1164 pathway pathway NN 1165 1167 by by IN 1168 1176 p21(ras) p21(ras) NNP 1177 1180 was be VBD 1181 1196 down-regulation down-regulation NN 1197 1199 of of IN 1200 1203 the the DT 1204 1212 activity activity NN 1213 1215 of of IN 1216 1219 the the DT 1220 1233 transcription transcription NN 1234 1240 factor factor NN 1241 1245 AP-1 ap-1 NN 1246 1249 and and CC 1250 1260 subsequent subsequent JJ 1261 1271 coordinate coordinate JJ 1272 1282 reductions reduction NNS 1283 1285 in in IN 1286 1290 IL-2 il-2 NN 1291 1295 gene gene NN 1296 1306 expression expression NN 1307 1310 and and CC 1311 1318 protein protein NN 1319 1329 production production NN 1329 1330 . . . 1331 1336 These these DT 1337 1344 results result NNS 1345 1352 suggest suggest VBP 1353 1357 that that IN 1358 1366 p2l(ras) p2l(ras) NN 1367 1369 is be VBZ 1370 1372 an an DT 1373 1382 essential essential JJ 1383 1391 mediator mediator NN 1392 1394 in in IN 1395 1405 generating generate VBG 1406 1409 not not RB 1410 1414 only only RB 1415 1423 positive positive JJ 1424 1427 but but CC 1428 1432 also also RB 1433 1441 negative negative JJ 1442 1452 modulatory modulatory JJ 1453 1463 mechanisms mechanism NNS 1464 1475 controlling control VBG 1476 1479 the the DT 1480 1490 competence competence NN 1491 1493 of of IN 1494 1495 T t NN 1496 1501 cells cell NNS 1502 1504 in in IN 1505 1513 response response NN 1514 1516 to to TO 1517 1526 inductive inductive JJ 1527 1539 stimulations stimulation NNS 1539 1540 . . .